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Tek fresh bedding

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Tek-Fresh bedding is a laboratory animal bedding product manufactured by Inotiv. It is designed to provide a clean and comfortable environment for laboratory animals. The core function of Tek-Fresh bedding is to absorb moisture and odors, while maintaining a dry and hygienic sleeping surface.

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Lab products found in correlation

Sprague dawley rats, by Inotiv (2 mentions) Teklad global 2920 soy protein free rodent diet, by Inotiv (1 mentions) C57bl 6j male mice, by Jackson ImmunoResearch (1 mentions) Rat chow, by Purina (1 mentions) C57bl 6j mice, by Jackson ImmunoResearch (1 mentions)

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TEK-Fresh (5 citations) TEK-Fresh cellulose bedding (2 citations)

13 protocols using tek fresh bedding

1

Antinociception Assay in Sprague-Dawley Rats

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Adult female Sprague–Dawley rats were used (60–90 days old, weighing 175–275 g) for the antinociception assay. Rats were bred in-house from Envigo stock (Livermore, CA USA), and housed under a 12:12 h light:dark cycle (lights on at approximately 6 a.m.) in a vivarium room maintained at 21 ± 2 °C. Rats were housed in pairs or triplets, and had ad libitum access to food and water except during testing; cages contained TekFresh® bedding (Envigo, Denver, CO USA). Rats were tested during the light part of the cycle, at approximately 8 a.m. – 1 p.m. ABK5 was dissolved in DMSO to which 1:1:8 ethanol:cremaphor:saline was added, for a final DMSO concentration of 10%; this DMSO solution was used as the vehicle for testing control rats. ABK5 was administered i.p. in a volume of 2 mL/kg. All procedures were approved by the Institutional Animal Care and Use Committee, Washington State University, and rats were treated in accordance with the National Research Council Guide for the Care and Use of Laboratory Animals (2011).
Tang Y., Wolk B., Britch S.C., Craft R.M, & Kendall D.A. (2020). Anti-inflammatory and antinociceptive effects of the selective cannabinoid CB2 receptor agonist ABK5. Journal of pharmacological sciences, 145(4), 319-326.
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2

Sprague Dawley Rat Acclimation and Surgery

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Forty experimentally naïve Sprague Dawley rats (n = 20 females, 20 males) from Envigo (Indianapolis, IN), approximately 9 weeks of age, were individually housed in clear polycarbonate tubs (35.5 x 32 x 18 cm; l x w x h) lined with TEK Fresh bedding (Envigo, Indianapolis, IN) in a temperature- and humidity-controlled colony room. We feel it important to include both sexes in all our studies—even when sex is not a primary factor of investigation—for matters of representation and external validity. Accordingly, males and females were equivalently represented in each group. However, exploring potential sex differences was not a goal of the present study and so the study was not powered to statistically examine the role of sex.
The rats had ad libitum access to food (Envigo, Teklad Global Diets®) and water over the week that they acclimated to the vivarium and during the week of recovery from surgery (see below). During the acclimation period, each rat was handled 2 min/day for the three consecutive days before surgery. Following 7 days of surgical recovery, food was restricted to maintain rats at 90% of free-feeding weight. All experimental sessions were conducted during the light phase of a 12-hr light/dark cycle. Protocols were approved by the University of Nebraska-Lincoln Institutional Animal Care and Use Committee.
Barrett S.T., Flynn A.T., Huynh W, & Bevins R.A. (2022). Appetitive Pavlovian conditioning of the stimulus effects of nicotine enhances later nicotine self-administration. Journal of the experimental analysis of behavior, 117(3), 543-559.
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3

Diurnal Rhythms and Leishmania Infection in Mice

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C57Bl/6 J mice were maintained at the Animal Research Facility, University of Northern Colorado, on a standard 12-h light/dark (L/D) cycle with lights on at 0700 h Mountain Standard Time (Zeitgeber time, ZT0) and lights off at 1900 h Mountain Standard Time (ZT12). In Experiment 1 (diurnal rhythmicity), mice were housed individually for a period of approximately 5 days in polypropylene shoebox-style cages that had been modified such that approximately 80% of the floor space was comprised of a galvanized wire mesh (1/8th inch mesh size -- sufficient to allow fecal pellets to pass through). In Experiment 2 (Leishmania infection), animals were housed individually in Optimice® cages containing Tek-Fresh bedding (#7099, Envigo). All animals were provided with food (Rodent Diet 2016, Envigo) and deionized water ad libitum. Animals were euthanized with an overdose of CO2.
Foxx C.L., Nagy M.R., King A.E., Albin D, & DeKrey G.K. (2022). TCDD exposure alters fecal IgA concentrations in male and female mice. BMC Pharmacology & Toxicology, 23, 25.
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4

Sprague-Dawley Rat Housing and Acclimatization

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Eight male Sprague–Dawley (SD) rats (Envigo, Indianapolis, IN, USA) were singly housed on Tek-Fresh bedding (Envigo) with environmental enrichment (PVC T-tubes and chew toys) and maintained in a 12:12-h light–dark (LD) cycle (lights on 7:00 a.m.–7:00 p.m.). Access to 16% rodent diet (Envigo) and tap water was provided ad libitum. SD rats were acclimatized to their housing environment at least 1 week prior to experimentation. All methods were approved by the Palmer College of Chiropractic Institutional Animal Care and Use Committee (IACUC).
Walter L.A., Blake L.P., Gallot Y.S., Arends C.J., Sozio R.S., Onifer S.M, & Bohnert K.R. (2021). Effect of Denervation on XBP1 in Skeletal Muscle and the Neuromuscular Junction. International Journal of Molecular Sciences, 23(1), 169.
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5

Sprague Dawley Rat Housing Protocol

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Thirty-four male Sprague Dawley rats (268–366 grams at the start of experimentation; Envigo, Indianapolis, IN, USA) were singly-housed on Tek-Fresh bedding (Envigo) with environmental enrichment (PVCT tube, chew toys) and maintained on a 12 hour light-dark cycle (lights on 7:00AM – 7:00PM). Access to 19% Rodent Diet (7433, Kent Feeds, Muscatine, IA) and tap water was provided ad libitum. Rats were acclimatized to their housing environment 1 week prior to experimentation. All methods were approved by the Palmer College of Chiropractic Institutional Animal Care and Use Committee.
Onifer S.M., Sozio R.S., DiCarlo D.M., Li Q., Donahue R.R., Taylor B.K, & Long C.R. (2018). Spinal Manipulative Therapy Reduces Peripheral Neuropathic Pain in the Rat. Neuroreport, 29(3), 191-196.
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6

Antinociception Assay in Sprague-Dawley Rats

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Adult female Sprague–Dawley rats were used (60–90 days old, weighing 175–275 g) for the antinociception assay. Rats were bred in-house from Envigo stock (Livermore, CA USA), and housed under a 12:12 h light:dark cycle (lights on at approximately 6 a.m.) in a vivarium room maintained at 21 ± 2 °C. Rats were housed in pairs or triplets, and had ad libitum access to food and water except during testing; cages contained TekFresh® bedding (Envigo, Denver, CO USA). Rats were tested during the light part of the cycle, at approximately 8 a.m. – 1 p.m. ABK5 was dissolved in DMSO to which 1:1:8 ethanol:cremaphor:saline was added, for a final DMSO concentration of 10%; this DMSO solution was used as the vehicle for testing control rats. ABK5 was administered i.p. in a volume of 2 mL/kg. All procedures were approved by the Institutional Animal Care and Use Committee, Washington State University, and rats were treated in accordance with the National Research Council Guide for the Care and Use of Laboratory Animals (2011).
Tang Y., Wolk B., Britch S.C., Craft R.M, & Kendall D.A. (2020). Anti-inflammatory and antinociceptive effects of the selective cannabinoid CB2 receptor agonist ABK5. Journal of pharmacological sciences, 145(4), 319-326.
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7

Sprague-Dawley Rat Behavioral Testing

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Adult Sprague-Dawley rats were bred in-house from Harlan stock (Livermore, CA): they were 60-89 days old on the first day of testing (body weight 184-272 g females, 239-431 g males).. Rats were housed under a 12:12 h light:dark cycle (lights on at 06.00 h) in a room maintained at 21±2°C, with TekFresh® bedding (Envigo, Denver, CO), with typically 2 same-sex rats/cage. Males and females were housed in the same room, but on different racks that were approximately 1.5 m apart, and rats had free access to food and water except during testing. Rats were randomly assigned to treatment groups with the exception that care was taken to avoid assigning same-sex siblings to the same treatment group. Rats were tested during the light part of the cycle, typically between 0900-1500 h, and were treated in accordance with the National Research Council Guide for the Care and Use of Laboratory Animals (2011) .
Craft R.M., Greene N.Z, & Wakley A.A. (2018). Antinociceptive effects of JWH015 in female and male rats. Behavioural pharmacology, 29(2-), 280-289.
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8

Sprague-Dawley Rat Model for Studies

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Adult (60–100 days old) male and female Sprague–Dawley rats were used (sample size/sex/group is reported below and in each figure legend). Rats were bred in-house from Envigo (formerly Harlan) stock (Livermore, California, USA), and housed under a 12:12-h light:dark cycle (lights on at 06:00) in a vivarium room maintained at 21 ± 2°C. Male and female rats were typically housed in same-sex pairs in the same vivarium room but with males and females on different racks. TekFresh bedding (Envigo, Denver, Colorado, USA) was used in the cages, and rats had ad libitum access to food and water except during testing. Rats were tested during the light part of the cycle, between 08:00 and 16:00. All procedures were approved by the Institutional Animal Care and Use Committee at Washington State University, and rats were treated in accordance with the National Research Council, Guide for the Care and Use of Laboratory Animals (2011) .
Craft R.M., Hewitt K.A, & Britch S.C. (2021). Antinociception produced by nonsteroidal anti-inflammatory drugs in female vs male rats. Behavioural pharmacology, 32(2-#x000263), 153-169.
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9

Prenatal Alcohol Exposure in Rats

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The University of New Mexico Health Sciences Center (UNM HSC) Institutional Animal Care and Use Committee (IACUC) approved all of the procedures involving the use of live rodents. All experiments were in compliance with the ARRIVE guidelines. The Long-Evans rats used in these studies were purchased from Envigo Corporation (Indianapolis, IN, USA). Upon arrival at our UNM HSC Animal Resource Facility, the rats were single housed in static micro isolator cages with Envigo Tek-Fresh bedding at 22°C on a reverse 12-h dark/12-h light schedule (lights on from 2100 to 0900 h). They were provided irradiated Teklad Global 2920 Soy Protein Free rodent diet (Envigo) and water (chlorine dioxide treated in water bottles, autoclaved prior to use) ad libitum. Female rats arrived at around 6–7 weeks of age (125–150 g) and were approximately 9–10 weeks old at the time of breeding. The males were established breeders, 12 weeks old upon arrival, and 15 to 16 weeks old at the start of the breeding protocol. After one-week acclimation to the animal facility, baseline body weights for each female were obtained prior to the start of the pre-pregnancy drinking phase of the PAE paradigm.
Davies S., Lujan K.S., Rappaport E.J., Valenzuela C.F, & Savage D.D. (2023). Effect of moderate prenatal ethanol exposure on the differential expression of two histamine H3 receptor isoforms in different brain regions of adult rat offspring. Frontiers in Neuroscience, 17, 1192096.
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10

Aging effects on mouse behavior

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Twenty-one adult 7.5-month-old (N = 8) and 10-month-old (N = 13) C57BL/6J male mice (Jackson Laboratories, Bar Harbor, ME) were provided for the study. These mice were excess mice donated from other projects within the testing facility, were raised in homecages for respective periods of time prior to testing, and were naïve to handling and other testing procedures for approximately 3 months. Animals were group-housed by age. Homecages were lined with TEKFresh bedding (Envigo, Indianapolis, IN) and contained a PVC pipe and nylabone as additional forms of enrichment. The colony was maintained on a 12-hour light/dark cycle (lights on at 8:00 a.m.) with unautoclaved 7012 diet feed (Envigo, Indianapolis, IN) and water offered ad libitum. Cages were washed and autoclaved once a week by husbandry staff.
Neely C., Lane C., Torres J, & Flinn J. (2018). The Effect of Gentle Handling on Depressive-Like Behavior in Adult Male Mice: Considerations for Human and Rodent Interactions in the Laboratory. Behavioural Neurology, 2018, 2976014.
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