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Multimode reader lb942

Manufactured by Berthold Technologies
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The Multimode Reader LB942 is a versatile laboratory instrument designed to perform various detection and measurement tasks. It is capable of detecting and quantifying a wide range of analytes, including fluorescent, luminescent, and absorbance-based assays. The LB942 is equipped with multiple detection modes, allowing users to select the appropriate method for their specific application.

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TriStar Multimode Reader LB942 TriStar2 Berthold/LB942 multimode reader Multimode reader LB942

2 protocols using multimode reader lb942

1

Luciferase Assay for Transcriptional Activity

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Agrobacterium bacteria carrying PGX3p-LUC and the 35S-EIN3 or 35S-Vector were prepared in infiltration buffer [10 mM MES (pH 5.6), 10 mM MgCl2, and 0.2 mM acetosyringone] at an optical density of 0.4. PGX3p-LUC was infiltrated into tobacco leaves with the 35S-EIN3 or 35S-Vector at a ratio of 1:1. After 2 days, 1 mM d-luciferin potassium salt solution (Biotium) was infiltrated, and luciferase bioluminescence images were obtained using a Berthold Technologies Multimode Reader LB942. For each combination, at least five infiltration spots were assessed. The bioluminescence intensity was quantified by Indigo software.
Wu Q., Li Y., Lyu M., Luo Y., Shi H, & Zhong S. (2020). Touch-induced seedling morphological changes are determined by ethylene-regulated pectin degradation. Science Advances, 6(48), eabc9294.
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2

Luciferase Complementation Imaging Assay

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The Luciferase Complementation Imaging (LCI) assay was carried out in tobacco leaves and Arabidopsis protoplasts as previously described (Chen et al., 2008 (link); Li et al., 2015 (link)), with minor modifications. Agrobacterium bacteria containing individual construct were suspended in the infiltration medium to a final concentration of OD600 = 0.4, and the two constructs in each pair were mixed equally. To avoid the bias of transfection efficiency, we used five individual tobacco plants. Biological triplicates were performed for every assay and the representative results were presented. Protoplasts were lysed from flat leaves of 4-week-old ein3eil1ebf1ebf2 mutant plants according to the protocol provided by Jen Sheen's laboratory (http://genetics.mgh.harvard.edu/sheenweb/). Then, 10 μg (<10 μl) of each plasmid was transferred to the protoplasts. The resultant luciferase activity was detected using a BERTHOLD TECHNOLOGIES Multimode Reader LB942.
Shi H., Shen X., liu R., Xue C., Wei N., Deng X.W, & Zhong S. (2016). The red light receptor phytochrome B directly enhances substrate-E3 ligase interactions to attenuate ethylene responses. Developmental cell, 39(5), 597-610.
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