Antibodies against lc3b

Silibinin was purchased from Sigma-Aldrich; Merck KGaA and dissolved to a final concentration of 50 mM in DMSO. Hydroxychloroquine sulfate (cat. no. H0915), 3-Methyladenine (3-MA; cat. no. M9281) and lithium chloride (LiCl; cat. no. L9650) were purchased from Sigma-Aldrich; Merck KGaA. Recombinant human transforming growth factor-β1 (TGF-β1; cat. no. 240-B) was purchased from R&D Systems, Inc., and used at a concentration of 5 ng/ml. Rabbit primary antibodies against vimentin (cat. no. 5471; 1:2,000), Wnt3a (cat. no. 2721; 1:500), phosphorylated β-catenin (Ser33/37; cat. no. 2009; 1:500), total β-catenin (cat. no. 8480; 1:1,000), phosphorylated glycogen synthase kinase (GSK)3β (Ser9; cat. no. 5558; 1:1,000), total GSK3β (cat. no. 12456; 1:1,000), autophagy-associated gene 5 (ATG5; cat. no. 9980; 1:1,000) and β-actin (cat. no. 4970; 1:5,000) were all purchased from Cell Signaling Technology, Inc. Antibodies against LC3B (cat. no. ab48394; 1:1,000), epithelial (E)-cadherin (cat. no. ab15148; 1:2,000), neural (N)-cadherin (cat. no. ab76057; 1:1,000), Histone H3 (cat. no. ab176842; 1:1,000) and GAPDH (cat. no. ab181602; 1:5,000) were purchased from Abcam.