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Cd24 fluorescein isothiocyanate fitc

Manufactured by BD
Sourced in United States

CD24/Fluorescein Isothiocyanate (FITC) is a lab equipment product that is used to detect and quantify the expression of the CD24 antigen on the surface of cells. Fluorescein Isothiocyanate (FITC) is a fluorescent dye that is conjugated to the CD24 antibody, allowing for the visualization and analysis of CD24-positive cells through flow cytometry or other fluorescence-based techniques.

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2 protocols using cd24 fluorescein isothiocyanate fitc

1

Isolation and Characterization of SCAP

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Human SCAP were isolated from an extracted immature mandibular third molar of a male patient aged 18 years old by a method used in a previous study (11 (link)). All protocols were reviewed and approved by the Ethics Committee of Guanghua School and Hospital of Stomatology, Sun Yat-sen University (Guangzhou, China). The osteogenic and adipogenic differentiation capacities of SCAP were identified by Alizarin Red staining and Oil Red O staining (Cyagen Biosciences, Inc., Guangzhou, China), respectively (11 (link)). The typical phenotypes, including STRO-1/Alexa Fluor 647-Allophycocyanin (BioLegend, Inc., San Diego, CA, USA), CD146/Phycoerythrin (BD Pharmingen, San Diego, CA, USA), CD24/Fluorescein Isothiocyanate (FITC) (BD Pharmingen) and CD45/FITC (BD Pharmingen), using the 2nd passage of SCAP were assessed using a FACSCalibur flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).
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2

Comprehensive B Cell Immunophenotyping

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Blood was collected in EDTA tubes, and 100 μl was incubated with anti-human CD19-eFluor450 (eBioscience, San Diego, CA, USA), CD24-fluorescein isothiocyanate (FITC) (BD biosciences, Franklin Lakes, NJ, USA), CD27-APC-eFluor780 (eBioscience), CD38-PeCy5 (eBioscience), CD5-PerCp-Cy5.5 (Biolegend, San Diego, CA, USA) or the corresponding isotype controls. After 15 minutes cells were treated with fluorescence-activated cell sorting (FACS) Lysing solution (BD Biosciences). Samples were measured using an LSR-II flow cytometer (BD biosciences) and data were analyzed using Kaluza 1.2 flow analysis software (Beckman Coulter, Brea, CA, USA). B cells were divided into transitional, memory, naive, CD24highCD38high and CD24highCD27+ B cells as described previously [14 (link)]. CD5+ B cells were gated on an isotype control.
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