Human SCAP were isolated from an extracted immature mandibular third molar of a male patient aged 18 years old by a method used in a previous study (11 (link)). All protocols were reviewed and approved by the Ethics Committee of Guanghua School and Hospital of Stomatology, Sun Yat-sen University (Guangzhou, China). The osteogenic and adipogenic differentiation capacities of SCAP were identified by Alizarin Red staining and Oil Red O staining (Cyagen Biosciences, Inc., Guangzhou, China), respectively (11 (link)). The typical phenotypes, including STRO-1/Alexa Fluor 647-Allophycocyanin (BioLegend, Inc., San Diego, CA, USA), CD146/Phycoerythrin (BD Pharmingen, San Diego, CA, USA), CD24/Fluorescein Isothiocyanate (FITC) (BD Pharmingen) and CD45/FITC (BD Pharmingen), using the 2nd passage of SCAP were assessed using a FACSCalibur flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).
Cd24 fluorescein isothiocyanate fitc
Manufactured by BD
Sourced in United States
CD24/Fluorescein Isothiocyanate (FITC) is a lab equipment product that is used to detect and quantify the expression of the CD24 antigen on the surface of cells. Fluorescein Isothiocyanate (FITC) is a fluorescent dye that is conjugated to the CD24 antibody, allowing for the visualization and analysis of CD24-positive cells through flow cytometry or other fluorescence-based techniques.
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Lab products found in correlation
Cd146 phycoerythrin, by BD (1 mentions)
Facscalibur flow cytometer, by BD (1 mentions)
Cd45 fitc, by BD (1 mentions)
Alizarin red staining, by Cyagen (1 mentions)
Oil red o, by Cyagen (1 mentions)
Cd27 apc efluor780, by Thermo Fisher Scientific (1 mentions)
Cd5 percp cy5, by BioLegend (1 mentions)
Anti human cd19 efluor450, by Thermo Fisher Scientific (1 mentions)
Kaluza 1.2 flow analysis software, by Beckman Coulter (1 mentions)
Facs lysing solution, by BD (1 mentions)
Lsrii flow cytometer, by BD (1 mentions)
2 protocols using cd24 fluorescein isothiocyanate fitc
1
Isolation and Characterization of SCAP
2
Comprehensive B Cell Immunophenotyping
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Blood was collected in EDTA tubes, and 100 μl was incubated with anti-human CD19-eFluor450 (eBioscience, San Diego, CA, USA), CD24-fluorescein isothiocyanate (FITC) (BD biosciences, Franklin Lakes, NJ, USA), CD27-APC-eFluor780 (eBioscience), CD38-PeCy5 (eBioscience), CD5-PerCp-Cy5.5 (Biolegend, San Diego, CA, USA) or the corresponding isotype controls. After 15 minutes cells were treated with fluorescence-activated cell sorting (FACS) Lysing solution (BD Biosciences). Samples were measured using an LSR-II flow cytometer (BD biosciences) and data were analyzed using Kaluza 1.2 flow analysis software (Beckman Coulter, Brea, CA, USA). B cells were divided into transitional, memory, naive, CD24highCD38high and CD24highCD27+ B cells as described previously [14 (link)]. CD5+ B cells were gated on an isotype control.
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