Rosa26 lsl l10gfp

All animal husbandry and procedures followed the ethical guidelines outlined in the NIH Guide for the Care and Use of Laboratory Animals (https://grants.nih.gov/grants/olaw/guide-for-the-care-and-use-of-laboratory-animals.pdf), and all protocols were approved by the institutional animal care and use committee (IACUC) at Harvard Medical School. Slc6a2-p2a-Cre and Gfral-p2a-Cre mice were constructed as described below; Glp1r-ires-Cre (Williams et al., 2016 (link)), Calcr-ires-Cre (Pan et al., 2018 (link)), and Slc17a6-ires-Cre (Vong et al., 2011 (link)) mice were described before; mouse lines for cell-specific deletion and rescue of Glp1r were gifts from Randy Seeley (University of Michigan) (Sisley et al., 2014 (link)); Calca-gfp mice were from GENSAT; wild-type C57BL/6J (000664), Rosa26-LSL-L10GFP (024750), Chat-gfp (007902), Rosa26-LSL-tdTomato (007908), Gad2-ires-Cre (010802), and Rosa26-LSL-DTR (007900) mice were purchased (Jackson Laboratory). Single-cell sequencing was performed on male C57BL/6J mice; both male and female mice between 8-24 weeks old were used for all other studies, and no differences based on sex were observed.

All animal husbandry and procedures were performed in compliance with institutional animal care and use committee guidelines. All animal husbandry and procedures followed the ethical guidelines outlined in the NIH Guide for the Care and Use of Laboratory Animals (https://grants.nih.gov/grants/olaw/guide-for-the-care-and-use-of-laboratory-animals.pdf), and all protocols were approved by the institutional animal care and use committee (IACUC) at Harvard Medical School. Slc6a2-p2a-Cre (Zhang et al., 2021 (link)), Gfral-p2a-Cre (Zhang et al., 2021 (link)), Glp1r-ires-Cre (Chang et al., 2015 (link); Williams et al., 2016 (link)), Calcr-ires-Cre (Pan et al., 2018 (link)), and Gipr-Cre (Adriaenssens et al., 2019 (link)) mice were described before; wild-type C57BL/6J (000664), Rosa26-lsl-L10GFP (024750), Rosa26-lsl-tdTomato (007908), Gad2-ires-Cre (010802), Agtr1a-t2a-Cre (031487), and Rosa26-lsl-DTR (007900) mice were purchased (Jackson Laboratory). Both male and female mice between 8–24 weeks old were used for all other studies, and no differences based on sex were observed.