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Polyclonal rat anti mouse igg

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Polyclonal rat anti-mouse IgG is a laboratory reagent used for the detection and quantification of mouse immunoglobulin G (IgG) in various immunoassays and research applications. It is produced by immunizing rats with mouse IgG, resulting in the generation of a diverse population of antibodies that recognize multiple epitopes on the target protein.

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Lab products found in correlation

Cytofix cytoperm fixation permeabilization kit, by BD (3 mentions) Live dead dye, by Thermo Fisher Scientific (3 mentions) Anti cd16 cd32, by BioXCell (3 mentions) Lsrfortessa, by BD (3 mentions) Foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (2 mentions) Mem non essential amino acids solution, by Thermo Fisher Scientific (1 mentions) Leukocyte activation cocktail, by BD (1 mentions) Glutamax supplement, by Thermo Fisher Scientific (1 mentions)

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Mouse anti-IgG (2 citations)

3 protocols using polyclonal rat anti mouse igg

1

Multiparameter Flow Cytometry Analysis

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Cells were stained with the antibodies listed in Supporting Information Additional Table S2, live/dead dye (Thermo Fisher Scientific) and anti-CD16/CD32 (Bio X Cell) for 20 min at 4 °C in the dark. All samples were fixed with 2% paraformaldehyde for 20 min. For detection of Vγ4+ cells, 20 µg of 1C10-1F7 antibody was used to stain the cells prior to secondary staining with a polyclonal rat anti-mouse IgG (Invitrogen). Cells were then stained with the other conjugated antibodies. For functional analysis, MLN cells were cultured at 37 °C, 5% CO2 for 4 h with BD Leukocyte Activation Cocktail (BD Pharmingen) in IMDM media containing 10% FBS, 10mM HEPES, 1mM sodium pyruvate, 2mM GlutaMAX™ supplement and 1X MEM non-essential amino acids solution (Thermo Fisher Scientific). Intracellular staining was performed using BD Cytofix/Cytoperm Fixation/Permeabilization kit (BD Biosciences) according to the manufacturer’s instructions. Stained cells were acquired on a LSRFortessa (BD Biosciences). Data were analyzed with FlowJo software (TreeStar).
Khairallah C., Chu T.H., Qiu Z., Imperato J.N., Yang D, & Sheridan B.S. (2022). The accumulation of Vγ4 T cells with aging is associated with an increased adaptive Vγ4 T cell response after foodborne Listeria monocytogenes infection of mice. Immunity & Ageing : I & A, 19, 19.
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2

Multiparametric Flow Cytometry Analysis

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For surface staining, cells were stained with the antibodies listed in Table S4, live/dead dye (Thermo Fisher Scientific) and anti-CD16/CD32 (Bio X Cell). For detection of Vγ4+ cells, cells were first stained with 20 μg of 1C10–1F7 antibody prior to secondary staining with a polyclonal rat anti-mouse IgG (Invitrogen). Cells were then stained with other antibodies. Intracellular cytokine staining was performed using BD Cytofix/Cytoperm Fixation/Permeabilization kit (BD Biosciences) according to the manufacturer’s instructions. Ki-67 detection was performed using eBioscience Foxp3/Transcription factor staining buffer set according to the manufacturer’s instructions (Invitrogen). For the detection of pTyr319/Tyr352 Zap-70/Syk, cells were sequentially stained with live/dead dye, anti-CD3ε and anti-TCRδ antibodies, fixed with 4% paraformaldehyde containing 1.5% methanol, permeabilized using 100% ice-cold methanol and finally stained with the remaining antibodies. All samples were acquired on a LSRFortessa (BD Biosciences). Data were analyzed with FlowJo software (TreeStar).
Khairallah C., Bettke J.A., Gorbatsevych O., Qiu Z., Zhang Y., Cho K., Kim K.S., Chu T.H., Imperato J.N., Hatano S., Romanov G., Yoshikai Y., Puddington L., Surh C.D., Bliska J.B., van der Velden A.W, & Sheridan B.S. (2021). A blend of broadly-reactive and pathogen-selected Vγ4Vδ1 T cell receptors confer broad bacterial reactivity of resident memory γδ T cells. Mucosal immunology, 15(1), 176-187.
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3

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
For surface staining, cells were stained with the antibodies listed in Table S4, live/dead dye (Thermo Fisher Scientific) and anti-CD16/CD32 (Bio X Cell). For detection of Vγ4+ cells, cells were first stained with 20 μg of 1C10–1F7 antibody prior to secondary staining with a polyclonal rat anti-mouse IgG (Invitrogen). Cells were then stained with other antibodies. Intracellular cytokine staining was performed using BD Cytofix/Cytoperm Fixation/Permeabilization kit (BD Biosciences) according to the manufacturer’s instructions. Ki-67 detection was performed using eBioscience Foxp3/Transcription factor staining buffer set according to the manufacturer’s instructions (Invitrogen). For the detection of pTyr319/Tyr352 Zap-70/Syk, cells were sequentially stained with live/dead dye, anti-CD3ε and anti-TCRδ antibodies, fixed with 4% paraformaldehyde containing 1.5% methanol, permeabilized using 100% ice-cold methanol and finally stained with the remaining antibodies. All samples were acquired on a LSRFortessa (BD Biosciences). Data were analyzed with FlowJo software (TreeStar).
Khairallah C., Bettke J.A., Gorbatsevych O., Qiu Z., Zhang Y., Cho K., Kim K.S., Chu T.H., Imperato J.N., Hatano S., Romanov G., Yoshikai Y., Puddington L., Surh C.D., Bliska J.B., van der Velden A.W, & Sheridan B.S. (2021). A blend of broadly-reactive and pathogen-selected Vγ4Vδ1 T cell receptors confer broad bacterial reactivity of resident memory γδ T cells. Mucosal immunology, 15(1), 176-187.
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