The negative epoxy based photoresist SU-8 was used for production of microcontainers. Formulations with two different viscosities were used (i.e., SU-8 2035 and 2075) and the cross-linked structures were developed in Developer mr-Dev 600. Resist and developer were purchased from Micro Resist Technology GmbH (Berlin, Germany). Single-side polished ø100 mm Si substrates with a thickness of 525 µm were acquired from Topsil Globalwafers A/S (Frederikssund, Denmark).
For the phosphate buffered saline (PBS) used in the in situ perfusion studies, sodium chloride, potassium chloride, sodium phosphate dibasic and potassium phosphate monobasic were purchased from Scharlab (Barcelona, Spain). Animals were from Charles River Laboratories (Quebec, QC, Canada). Sylgaard 184 silicone elastomer kit was purchased from Dow Chemical (Midland, MI, USA). Ultrapure water used throughout the studies was obtained from a Q-POD® dispenser (Merck Millipore, Burlington, MA, USA).