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2 protocols using sodium phosphate dibasic

1

Microcontainer Fabrication and Perfusion Studies

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Amoxicillin trihydrate was bought from TCI (Tokyo, Japan) and Eudragit® L100 was acquired from Evonik Industries (Essen, Germany). Dibutyl sebacate, isopropanol and potassium phosphate monobasic for the HPLC mobile phase were purchased from Sigma Aldrich (St. Louis, MO, USA). Methanol was bought from VWR International (Radnor, PA, USA).
The negative epoxy based photoresist SU-8 was used for production of microcontainers. Formulations with two different viscosities were used (i.e., SU-8 2035 and 2075) and the cross-linked structures were developed in Developer mr-Dev 600. Resist and developer were purchased from Micro Resist Technology GmbH (Berlin, Germany). Single-side polished ø100 mm Si substrates with a thickness of 525 µm were acquired from Topsil Globalwafers A/S (Frederikssund, Denmark).
For the phosphate buffered saline (PBS) used in the in situ perfusion studies, sodium chloride, potassium chloride, sodium phosphate dibasic and potassium phosphate monobasic were purchased from Scharlab (Barcelona, Spain). Animals were from Charles River Laboratories (Quebec, QC, Canada). Sylgaard 184 silicone elastomer kit was purchased from Dow Chemical (Midland, MI, USA). Ultrapure water used throughout the studies was obtained from a Q-POD® dispenser (Merck Millipore, Burlington, MA, USA).
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2

Corneal Drug Delivery Protocol

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CPXb was bought from Acros Organics (New Jersey, USA), and CPXs was acquired from Cayman Chemical Company (Michigan, USA). Soluplus was kindly donated by BASF SE (Ludwigshafen, Germany). Poly-vinyl alcohol (PVA), polycaprolactone (PCL), gelatin from bovine skin, and chitosan were purchased from Sigma-Aldrich (Missouri, USA), and propylene glycol (PG), polyethylene glycol (PEG), hydroxy ethyl cellulose (HEC), and hydroxy propyl methyl cellulose (HPMC) were purchased from Fagron Iberica (Barcelona, Spain). Organic solvents such as DMSO and chloroform were purchased from Sigma-Aldrich (Missouri, USA). For the phosphate-buffered saline (PBS) used, sodium chloride, potassium chloride, sodium phosphate dibasic, and potassium phosphate monobasic were purchased from Scharlab (Barcelona, Spain). Ultrapure water used throughout the studies was obtained from a Q-POD dispenser (Merck Millipore, Burlington, MA, USA). Acetonitrile for the HPLC mobile phase was purchased VWR International (Radnor, PA, USA). Dialysis membranes (Spectra/Por molecular porous membrane tubing) for in vitro release studies were acquired from Repligen (MA, USA). Corneas were from porcine eyes procured from a local slaughterhouse Mercavalencia (Valencia, Spain).
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