After drying and spraying gold, the sand column samples were tested by SEM to observe their microscopic morphology and structure. The SEM test equipment adopted a Sigma 300 field emission scanning electron microscope produced by Zeiss, Germany, and the test acceleration voltage was 10 or 15 kV. Samples were taken for XRD testing to compare and analyse the change in material composition in calcareous sand before and after biostimulated mineralization. The equipment used in the XRD test was a SmartLab produced by Rigaku Corporation, Japan. The working voltage of the sample test was 40 kV, the current was 30 mA, the angle parameters of the XRD test were set to 5~60°, and the step size was 0.02.
Sigma 300 field emission scanning electron microscope
Manufactured by Zeiss
Sourced in Germany, United Kingdom
The Sigma 300 field emission scanning electron microscope is a high-performance imaging and analysis instrument. It utilizes a field emission electron source to produce a fine, high-intensity electron beam. The microscope is designed to provide high-resolution imaging and analytical capabilities for a wide range of sample types and applications.
Automatically generated - may contain errors
8 protocols using sigma 300 field emission scanning electron microscope
1
Biostimulated Mineralization of Calcareous Sand
2
Scanning electron microscopy of rat brain ventricles
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Six rats on P21 were perfused with PBS, followed by a mixture of 2.5% glutaraldehyde/2% PFA/0.1 M sodium cacodylate/HCl buffer pH 7.2. Anterior and posterior areas of the lateral or medial walls of the lateral ventricles were micro-dissected and fixed overnight. Anatomically matched tissues were incubated in 1% osmium tetroxide in 0.1 sodium cacodylate/HCl buffer for 4 h, dehydrated in a series of graded ethanol solutions, carbon-coated, then imaged in a Zeiss Sigma 300 field emission scanning electron microscope, consistent with published reports (Sawamoto et al., 2006 (link); Xiong et al., 2014 (link)).
3
Hydrogel Surface Characterization Protocol
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The surface roughness of the membranes was observed using a laser scanning confocal microscope profilometer (LSM700, Zeiss, Germany). Sa (the arithmetic average height deviation from the mean plane) was selected to describe the surface roughness of hydrogels. The water contact angle was measured using a DSA-X ROLL contact angle measuring instrument. The profile of a drop of 2 μL of deionized water on the surface of hydrogels was captured. The intersection angles of both sides were measured to calculate average values. SEM and EDS were used to observe the cross-section morphology and elemental composition of lyophilized hydrogels. All samples were sputter-coated with gold layers and then analyzed using a Zeiss Sigma 300 field-emission Scanning Electron Microscope. The obtained SEM images were evaluated using Image J software to analyze the average pore diameters. Meanwhile, scanning maps of B, C, O, N, Mg were also measured by EDS.
4
Characterization of MoS2 and MoO3 Films
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A Zeiss Sigma
300 field emission scanning electron microscope was used to investigate
the morphological characteristics of MoS2 and MoO3 films. A WITech alpha 300R was used for micro-Raman measurements
of MoS2, MoS2/MoO3, and MoO3 films. For crystal structure characterization of films, a PANalytical
Empyrean X-ray diffraction (XRD) system was used. X-ray photoelectron
spectroscopy (XPS) measurements for determining the material composition
were performed with a Specs Flex-Mod XPS system equipped with a 150
mm radius hemispherical energy analyzer with a 2D charge-coupled detector.
The measurements were performed using an Al anode with a Kα energy of 1486.71 eV. The depth profile measurements were conducted
by 3 keV Ar+ ion sputtering with 300 s of etching for each
cycle.
300 field emission scanning electron microscope was used to investigate
the morphological characteristics of MoS2 and MoO3 films. A WITech alpha 300R was used for micro-Raman measurements
of MoS2, MoS2/MoO3, and MoO3 films. For crystal structure characterization of films, a PANalytical
Empyrean X-ray diffraction (XRD) system was used. X-ray photoelectron
spectroscopy (XPS) measurements for determining the material composition
were performed with a Specs Flex-Mod XPS system equipped with a 150
mm radius hemispherical energy analyzer with a 2D charge-coupled detector.
The measurements were performed using an Al anode with a Kα energy of 1486.71 eV. The depth profile measurements were conducted
by 3 keV Ar+ ion sputtering with 300 s of etching for each
cycle.
5
Scanning Electron Microscopy of Organoids
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Organoids were fixed with 2.5% (v/v) glutaraldehyde and 0.05 M cacodylate buffer (pH 7.2) in PBS overnight at 4 °C. 8 μL samples spotted onto a silicon disc coated with 0.01% (w/v) poly-l-lysine. Discs were washed twice with PBS and dehydrated with a graded ethanol series (30%, 50%, 70%, 80%, 100% ethanol (v/v), for 2 h each at 4 °C and 100% EtOH, for overnight). Following dehydration, samples were immersed for 5 min in pure hexamethyldisilazane (HDMS) and air dried. All specimens were mounted on aluminium stubs using double-sided carbon tape and then were sputter coated with 15 nm gold in a sputter coater (Quorum Technologies, Laughton, East Sussex, UK) and observed under a Zeiss Sigma 300 Field-Emission scanning electron microscope (ZEISS). Materials are listed in Supplementary Table 13.
6
Examination of Vitrified Tissue Surfaces
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The SEM images were taken from the vitrified tissue as it was, so as not to affect its integrity. Indeed, the samples being non-conductive, applying a metallic coating would have altered the surface characteristics. SEM imaging was performed by a Zeiss Sigma 300 Field Emission Scanning Electron Microscope (FESEM) (Department of Science, Università Roma Tre). Variable operational conditions (e.g., accelerating voltage) are indicated within each image.
7
Characterizing Crosslinked Scaffold Pores
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For scanning electron microscopy, crosslinked scaffolds were washed in demineralized water to remove storage medium, and subsequently frozen at −80 °C. The frozen scaffolds were lyophilized, mounted on stubs with double-sided carbon tape, and coated with an ultrathin gold layer (Scancoat Six Sputter Coater, Edwards, Crawley, United Kingdom). The samples were evaluated with a Sigma 300 field emission scanning electron microscope (Carl Zeiss B. V., Sliedrecht, The Netherlands) with an accelerating voltage of 10 kV. The pore sizes of the different scaffolds were determined using ImageJ analysis software.
8
Nanoparticle Characterization by SEM and TEM
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Scanning electron microscopy is performed using
a Sigma 300 field emission scanning electron microscope, Ziess, fitted
with in-lens SE, SE (secondary electron), and BS (backscatter electron)
detectors. The samples are loaded on a carbon tape, and the data are
analyzed using SmartSEM software. High-resolution transmission electron
microscopy (HRTEM) images are recorded using JEOL and JEM 2100 to
confirm the size, morphology and crystallinity of the nanoparticles,
and energy-dispersive X-ray spectroscopy (EDX) mapping is done to
assess the composition of the samples. The samples are prepared using
the drop cast method on a copper-coated carbon grid and dried in an
oven at 60 °C.
a Sigma 300 field emission scanning electron microscope, Ziess, fitted
with in-lens SE, SE (secondary electron), and BS (backscatter electron)
detectors. The samples are loaded on a carbon tape, and the data are
analyzed using SmartSEM software. High-resolution transmission electron
microscopy (HRTEM) images are recorded using JEOL and JEM 2100 to
confirm the size, morphology and crystallinity of the nanoparticles,
and energy-dispersive X-ray spectroscopy (EDX) mapping is done to
assess the composition of the samples. The samples are prepared using
the drop cast method on a copper-coated carbon grid and dried in an
oven at 60 °C.
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