To image flaxseed mucilage morphology, one seed was placed in a 24-well plate stained in 0.01% ruthenium red and imaged with a DP74 camera (Olympus) mounted on an Axio Imager M1 compound microscope (Zeiss) at specific time points. To image RKN that entered the flaxseed seedlings, infected seedlings were stained with acid fuchsin as described (65 (link)). Stained seedlings were photographed with a DP74 camera (Olympus) mounted on an Axio Zoom V16 dissecting microscope (Zeiss).
Axio zoom v16 dissecting microscope
The Axio Zoom V16 is a dissecting microscope designed by Zeiss. It features a zoom range of 0.8x to 16x, allowing for a wide range of magnification options. The microscope is equipped with advanced optics and illumination systems to provide clear, high-quality images for a variety of applications.
4 protocols using axio zoom v16 dissecting microscope
Microscopic Analysis of Plant-Nematode Interactions
To image flaxseed mucilage morphology, one seed was placed in a 24-well plate stained in 0.01% ruthenium red and imaged with a DP74 camera (Olympus) mounted on an Axio Imager M1 compound microscope (Zeiss) at specific time points. To image RKN that entered the flaxseed seedlings, infected seedlings were stained with acid fuchsin as described (65 (link)). Stained seedlings were photographed with a DP74 camera (Olympus) mounted on an Axio Zoom V16 dissecting microscope (Zeiss).
Microscopic Imaging of Nematode Attraction
For imaging of mucilage morphology, $20 seeds were treated or digested as described above, stained in 0.01% RR for 1 h, and washed once with 1 ml of ddH 2 O before being photographed with a DP74 camera (Olympus) mounted on an Axio Imager M1 compound microscope (Zeiss). To assay the number of RKNs that had infiltrated Arabidopsis Molecular Plant 12, 99-112, January 2019 ยช The Author 2018. 109
Seed Coat Secretes Root-Knot Nematode Attractant
Molecular Plant seedlings, we stained infected seedlings with acid fuchsin as described by Byrd et al. (1983) . Stained seedlings were photographed with a DP74 camera (Olympus) mounted on an Axio Zoom V16 dissecting microscope (Zeiss), and the number of infiltrated RKNs per seedling was counted.
Microscopic Imaging of Plant Seedlings
Nematode Attraction Imaging
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