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Abe257

Manufactured by Fortis Life Sciences
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The ABE257 is a laboratory equipment product. It is used for general laboratory applications. No further details are available.

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Lab products found in correlation

Fluorescence mounting medium, by Agilent Technologies (2 mentions) Alexa fluor 488 donkey anti rabbit antibody, by Thermo Fisher Scientific (2 mentions) Cultureslide, by BD (2 mentions) Anti phospho rpa32, by Fortis Life Sciences (2 mentions) Anti phospho histone h2ax, by Fortis Life Sciences (2 mentions) Tcs spe 2, by Leica camera (2 mentions)

2 protocols using abe257

1

Immunofluorescence analysis of DNA damage response in organoids

Check if the same lab product or an alternative is used in the 5 most similar protocols
Untreated and HU-treated (at a concentration of 2.5 mmol/L for 24 hours) organoids, grown on chamber slides (Falcon Culture-Slides) previously precoated with BME, were fixed in 4% paraformaldehyde in PBS solution for 30 minutes at room temperature and permeabilized with 0.5% Triton-X100 in PBS for 30 minutes at room temperature. Organoids were then incubated with 1% BSA in PBS for 60 minutes, followed by incubation overnight with the following primary antibodies diluted in PBS containing 1% of BSA and 1% of donkey serum: anti-RAD51 (Millipore ABE257; 1:100), anti-phospho-RPA32 (Ser33; Bethyl Laboratories A300-246A; 1:500), and anti-phospho-Histone H2AX (Ser139; Bethyl Laboratories A300-081A; 1:600). After washing, organoids were fluorescently labeled with Alexa Fluor 488 donkey anti-rabbit antibody (Invitrogen) diluted 1:400 in PBS containing 1% BSA and 1% donkey serum for 1 to 2 hours. Nuclei were stained with DAPI. Slides were then mounted using the fluorescence mounting medium (Dako) and analyzed using a confocal laser scanning microscope (TCS SPE II, Leica).
Durinikova E., Reilly N.M., Buzo K., Mariella E., Chilà R., Lorenzato A., Dias J.M., Grasso G., Pisati F., Lamba S., Corti G., Degasperi A., Cancelliere C., Mauri G., Andrei P., Linnebacher M., Marsoni S., Siena S., Sartore-Bianchi A., Nik-Zainal S., Di Nicolantonio F., Bardelli A, & Arena S. (2022). Targeting the DNA Damage Response Pathways and Replication Stress in Colorectal Cancer. Clinical Cancer Research, 28(17), 3874-3889.
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2

Immunofluorescence analysis of DNA damage response in organoids

Check if the same lab product or an alternative is used in the 5 most similar protocols
Untreated and HU-treated (at a concentration of 2.5 mmol/L for 24 hours) organoids, grown on chamber slides (Falcon Culture-Slides) previously precoated with BME, were fixed in 4% paraformaldehyde in PBS solution for 30 minutes at room temperature and permeabilized with 0.5% Triton-X100 in PBS for 30 minutes at room temperature. Organoids were then incubated with 1% BSA in PBS for 60 minutes, followed by incubation overnight with the following primary antibodies diluted in PBS containing 1% of BSA and 1% of donkey serum: anti-RAD51 (Millipore ABE257; 1:100), anti-phospho-RPA32 (Ser33; Bethyl Laboratories A300-246A; 1:500), and anti-phospho-Histone H2AX (Ser139; Bethyl Laboratories A300-081A; 1:600). After washing, organoids were fluorescently labeled with Alexa Fluor 488 donkey anti-rabbit antibody (Invitrogen) diluted 1:400 in PBS containing 1% BSA and 1% donkey serum for 1 to 2 hours. Nuclei were stained with DAPI. Slides were then mounted using the fluorescence mounting medium (Dako) and analyzed using a confocal laser scanning microscope (TCS SPE II, Leica).
Durinikova E., Reilly N.M., Buzo K., Mariella E., Chilà R., Lorenzato A., Dias J.M., Grasso G., Pisati F., Lamba S., Corti G., Degasperi A., Cancelliere C., Mauri G., Andrei P., Linnebacher M., Marsoni S., Siena S., Sartore-Bianchi A., Nik-Zainal S., Di Nicolantonio F., Bardelli A, & Arena S. (2022). Targeting the DNA Damage Response Pathways and Replication Stress in Colorectal Cancer. Clinical Cancer Research, 28(17), 3874-3889.
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