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Ab66155 is a primary antibody product offered by Cell Signaling Technology. It is a tool used for research purposes.

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Lab products found in correlation

Ab75853, by Abcam (1 mentions) Histoclear, by National Diagnostics (1 mentions)

2 protocols using ab66155

1

Immunohistochemical Analysis of Apoptosis and Proliferation

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Post experiment, rats were perfuse-fixed with 4% paraformaldehyde and embedded in paraffin. Brain tissue sections (6 µm) were immunohistochemically stained for Ki-67 and cleaved Caspase-3 as previously described20 (link). Primary antibody diluted in 1% BSA/PBS was applied overnight at 4 °C for Ki-67 (1:25, Abcam, ab66155) and Caspase-3 (1:25, Cell Signaling Technology, #9661). The sections were incubated with a goat anti-rabbit secondary antibody (1:500, Pierce, #31460) for 60 minutes followed by incubation with metal enhanced 3,3-diaminobenzidine (DAB; Life technologies, #34065) for 10 min. Expression of immunohistochemical markers was quantified by evaluating the presence of DAB staining and visually quantifying positive staining.
Rao J.U., Coman D., Walsh J.J., Ali M.M., Huang Y, & Hyder F. (2017). Temozolomide arrests glioma growth and normalizes intratumoral extracellular pH. Scientific Reports, 7, 7865.
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2

Immunohistochemical Analysis of Tissue Samples

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Samples were placed in 10% formalin buffer for 24 hours and then transferred to ethanol and embedded in paraffin. Paraffin embedded tissues were de-paraffinized using HistoClear (National diagnostics). Slides were then boiled in pH 6.0 citric buffer for antigen retrieval. Morphological analysis was peformed using ImageJ (Research Services Branch, National Institutes of Health, Bethesda, MD).
Tissue lysate preparation, SDS-PAGE and Western blotting was preformed as previously described (Saeidi et al., 2013 (link)). The following antibodies were used: GHR (1:50, Abcam 202964), UCP1 (1:100, ThermoFisher Scientific, PA5–29575), PPARα (1:100, Abcam 8934), Il33 (1:50 R&D Systems af3626), Reg3b (1:100, ThermoFisher Scientific, AF5110), MYH7 (1:100, Abcam 11083), HMGCR (1:250, Biovision 3952–100), CYCE (1:1000, Cell Signaling 4129), CDC2 (1:1000, Cell Signaling 9112), HK2 (Cell Signaling 2867), phosphoPDK1 (1:1000, Cell Signaling C49H2), Ki67 (1:250, Abcam ab66155), phospho4EBP1 (1:1000, Cell Signaling, 236B4), B2M (1:1000, Abcam ab75853). Tissues were counterstained with DAPI or Hematoxylin.
Ben-Zvi D., Meoli L., Abidi W.M., Nestoridi E., Panciotti C., Castillo E., Pizarro P., Shirley E., Gourash W.F., Thompson C.C., Munoz R., Clish C.B., Anafi R.C., Courcoulas A.P, & Stylopoulos N. (2018). Time-Dependent Molecular Responses Differ Between Gastric Bypass and Dieting But Are Conserved Across Species. Cell metabolism, 28(2), 310-323.e6.
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