Ferric 3 chloride hexahydrate

Standards of tryptophan, serotonin, and melatonin, as well as the Bis-Tris reagent, ABTS^•+, 2,4,6-tripyridyl-s-triazine (TPTZ), fluorescein, 2,2′-azobis(2-methylpropionamidine) (AAPH), ferric (III) chloride hexahydrate, manganese (IV) oxide, and the standard 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) were obtained from Sigma-Aldrich (Steinheim, Germany). The 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) reagent was purchased from Chemos GmbH (Regenstauf, Germany). Formic acid, hydrochloric acid (37%), dipotassium hydrogen phosphate, sodium acetate, and ammonium acetate were purchased from Panreac Quimica S.A. (Castellar del Vallés, Barcelona, Spain). Boric acid was purchased from Probus (Badalona, Barcelona, Spain). All LC-MS-grade solvents were obtained from JT Baker (Phillipsburg, NJ, USA). Ultrapure water was produced using a Millipore water purification system (Darmstadt, Germany).

Folin-Ciocalteau (FC) reagent, sodium carbonate, sodium nitrite, aluminium chloride, sodium hydroxide, sodium acetate trihydrate, acetic acid, 2,4,6-tripyridyl-s-triazine (TPTZ), ferric (III) chloride hexahydrate, vanillin, sulphuric acid and assay standards, including trolox, gallic acid, rutin and aecsin, were purchased from Sigma (Castle Hill, NSW, Australia). Ethanol was purchased from Fronine (Taren Point, NSW, Australia). The encapsulating agents, MD (DE 18) and GA, were purchased from the Melbourne Food Depot (Brunswick East, Vic, Australia). Deionised water (DI) was prepared fresh before use using a Millipore Milli-Q water purification system (North Ryde, NSW, Australia).

Methanol and acetone were obtained from Merck (Kilsyth, VIC, Australia). Ethanol was purchased from Fronine (Taren Point, NSW, Australia). Hydrochloric acid (36%) was obtained from Ajax Finechem (North Ryde, NSW, Australia) and n-butanol was purchased from Swift Australia Chemical Supplier (Mulgrave, VIC, Australia). Folin-Ciocalteau (FC) reagent, 2,2′-azinobis-(3-ethylbenzothiozoline-6-sulfonic acid (ABTS), 2,2′-diphenyl-1-picrylhydrazyl (DPPH), sodium carbonate, sodium nitrite, aluminium chloride, sodium hydroxide, vanillin, sodium acetate trihydrate, acetic acid, 2,4,6-tripyridyl-s-triazine (TPTZ), ferric (III) chloride hexahydrate, sulphuric acid and standards (trolox, gallic acid, rutin and aecsin) were purchased from Sigma-Aldrich (Castle Hill, NSW, Australia).