Peptide synthesis reagents and Fmoc-protected amino acids were purchased from Merck Biosciences (Darmstadt, Germany) and/or Iris Biotech (Marktretwitz, Germany). 15N-labelled amino acids were purchased from Cambridge Isotope Laboratories (Andover, MA, USA) and were Fmoc-protected using Fmoc-Cl as described previously69 . Solvents for peptide synthesis were purchased from Merck (Darmstadt, Germany) or from Biosolve (Valkenswaard, Netherlands) and solvents for HPLC purification were obtained from Fischer Scientific (Geel, Belgium). The lipids 1,2-dierucoyl-sn-glycero-3-phosphatidylcholine (DErPC), 1,2-dierucoyl-sn-glycero-3-phosphatidylglycerol (DErPG), 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC), 1,2-dimyristoyl-sn-glycero-3-phosphatidylglycerol (DMPC), 1,2-dimyristoleoyl-sn-glycero-3-phosphatidylcholine (DMoPC), 1,2-dioleoyl-sn-glycero-3-phosphatidylglycerol (DOPG), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylglycerol (POPG), and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rhod-PE) were obtained from Avanti Polar Lipids (Alabaster, USA). The fluorescent probes 8-amino-naphtalene-1,3,6-trisulfonic acid sodium salt (ANTS) and pxylenebis(pyridinium)bromide (DPX) were obtained from Invitrogen - Molecular Probes (Karlsruhe, Germany).
15n labelled amino acids
Manufactured by Cambridge Isotopes
Sourced in United States
15N-labelled amino acids are a type of laboratory equipment used in various research and analytical applications. These compounds are amino acids that have been chemically modified to incorporate a stable isotope of nitrogen (15N) instead of the more common 14N isotope. This allows researchers to track the movement and incorporation of these amino acids in biological systems using specialized analytical techniques, such as mass spectrometry. The core function of 15N-labelled amino acids is to serve as a tool for studying protein synthesis, metabolism, and other biological processes.
Automatically generated - may contain errors
Lab products found in correlation
1 2 dioleoyl sn glycero 3 phosphoethanolamine n lissamine rhodamine b sulfonyl rhod pe, by Avanti Polar Lipids (2 mentions)
1 palmitoyl 2 oleoyl sn glycero 3 phosphatidylcholine, by Avanti Polar Lipids (1 mentions)
1 palmitoyl 2 oleoyl sn glycero 3 phosphatidylglycerol, by Avanti Polar Lipids (1 mentions)
1 2 dimyristoyl sn glycero 3 phosphatidylcholine, by Avanti Polar Lipids (1 mentions)
Fmoc protected amino acids, by Merck Group (1 mentions)
P xylene bis pyridinium bromide dpx, by Thermo Fisher Scientific (1 mentions)
8 amino naphtalene 1 3 6 trisulfonic acid sodium salt ants, by Thermo Fisher Scientific (1 mentions)
2 protocols using 15n labelled amino acids
1
Peptide Synthesis and Lipid Preparation
2
Synthesis and Labeling of Lipids
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15N-labelled amino acids were purchased from Cambridge Isotope Laboratories (Andover, MA, USA) and were Fmoc-protected using Fmoc-Cl as described previously [33 (link)]. The lipids 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC), 1,2-dimyristoyl-sn-glycero-3-phosphatidylglycerol (DMPG), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC), and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylglycerol (POPG), were purchased from NOF Europe (Grobbendonk, Belgium). The fluorescent lipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rhod-PE) were obtained from Avanti Polar Lipids. The fluorescent probes 8-amino-naphtalene-1,3,6-trisulfonic acid sodium salt (ANTS) and p-xylene-bis(pyridinium)bromide (DPX) were obtained from Invitrogen-Molecular Probes (Karlsruhe, Germany).
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