Agilent workbench software

After obtaining blood from the patient and his parents, lymphocytes were extracted, and lymphoblasts were established by Epstein–Barr virus. Array CGH analysis was performed using the Agilent Human genome CGH 180K (Agilent Technologies, Santa Clara, CA, USA). In brief, DNA was isolated from the lymphocytes, using the DNeasy Blood and Tissue kit (Qiagen, Hilden, Germany), according to the manufacturer's instructions. Genomic DNA (1 μg) from the patient and reference DNA samples from unaffected volunteers (Coriell Institute for Medical Research, Camden, New Jersey, USA) were labeled with fluorescent dyes (Cy5-dUTP and Cy3), hybridized at 65 °C for 24 hours, and then subjected to the array. The hybridized array was scanned with an Agilent DNA Microarray Scanner (Agilent Technologies). The resulting images were analyzed by quantifying the Cy3 and Cy5 fluorescence intensity at each feature on the array, using the Agilent Feature Extraction Software (Agilent Technologies). Finally, the data were calculated using the Agilent Workbench Software (Agilent Technologies).