F4 80
The F4/80 is a murine macrophage-specific antibody that recognizes a 160 kDa glycoprotein expressed on the surface of mature macrophages. It is commonly used in flow cytometry and immunohistochemistry applications to identify and characterize macrophage populations.
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12 protocols using f4 80
Quantifying Colon Inflammation via F4/80 Immunohistochemistry
Histological Analysis of Post-MI Inflammation
Cryosectioning and Immunostaining of Enucleated Eyeballs
Molecular Mechanisms of Anti-Inflammatory Actions
Murine Immune Cell Phenotyping
Quantifying Wound Angiogenesis and Inflammation
Immunofluorescence Analysis of Pancreatic Tissues
Immunocytochemistry and Immunohistochemistry of Spinal Cord Injury
For immunohistochemistry, slides of injured spinal segments were blocked in 0.01 M PBS containing 3% BSA and 0.1% Triton X‐100 for 20 min. Subsequently, slides were incubated with primary antibodies: Iba1 (019‐19741, 1:1000; Wako), iNOS (ab49999, 1:200; Abcam), Arginase1 (sc‐166920, 1:200; Santa Cruz Biotechnology), GFAP (1:2000; Sigma), NeuN (#24307, 1:200; Cell Signaling Technology) at RT overnight. The slides were then stained with corresponding secondary antibodies, and counterstained with Hoechst 33342 to display nuclei. Slides were photographed by a confocal microscope (LSM 800; Zeiss).
Quantification of Iron Homeostasis Markers
Fluorescent Immunohistochemistry for Cell Identification
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