The following anti-human antibodies were used in this study: anti-CD3-BV605 (clone OKT3, Biolegend), anti-CD3-BUV395 (clone UCHT1, BD Bioscience), anti-CD14-V500 (clone M5E2, BD Bioscience), anti-CD19-BV510 (clone SJ25C1, BD Bioscience), anti-CD56-PE-Cy7 (clone NCAM16.2, BD Bioscience), anti-CXCR6-PE or anti-CXCR6-APC (clone K041E5, Biolegend), anti-CD57-AF488 (clone TB01, eBioscience), anti-TRAIL-BV421 (clone RIK-2, BD Bioscience), anti-CD49a-FITC (clone TS2/7, Biolegend), anti-HLA-DR V500 (clone G46-6, BD Bioscience) and anti-CD69-PE-Dazzle (clone FN50, Biolegend) for surface antigens; and anti-T-bet-eFlour610 (clone 4B10, eBioscience) and anti-Eomes-PE-eFlour610 (clone Dan11mag, eBioscience) for intranuclear antigens; anti-IFNγ-V450 (BD Bioscience), anti-MIP-1β-PerCP-Cy5.5 (cloneD21–1351, BD Bioscience), anti-GM-CSF-PE-CF594 (clone BVD2–21C11, BD Bioscience), anti-TNF-FITC (clone MAb11, BD Bioscience), anti-Granzyme B-AlexaFlour700 (clone GB11, BD Bioscience) and anti-Perforin-PerCP-Cy5.5 (clone dG9, Biolegend) for intracellular antigens.
Anti cd3 bv605 clone okt3
Manufactured by BioLegend
Anti-CD3-BV605 (clone OKT3) is a monoclonal antibody that recognizes the CD3 complex on human T cells. It is conjugated with the fluorescent dye BV605.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd56 pe cy7 clone ncam16, by BD (1 mentions)
Anti mip 1β percp cy5, by BD (1 mentions)
Anti cd3 buv395 clone ucht1, by BD (1 mentions)
Anti ifn γ v450, by BD (1 mentions)
Anti ccr7 fitc clone 150503, by BD (1 mentions)
Lsrfortessa system, by BD (1 mentions)
Live dead fixable violet, by Thermo Fisher Scientific (1 mentions)
2 protocols using anti cd3 bv605 clone okt3
1
Comprehensive Immune Profiling using Multicolor Flow Cytometry
2
Comprehensive T-cell Phenotyping Protocol
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T-cell differentiation was assessed using the following antibodies: anti-CCR7–FITC clone 150503 (BD Pharmingen); anti-CD45RO–PE clone UCHL1 and anti-CD8–H7APC clone SK1 (BD Biosciences); and anti-CD4–BV510 clone OKT4, anti-CD3–BV605 clone OKT3, anti-CD14–Pacific Blue clone HCD14 and anti-CD19–Pacific Blue clone H1B19 (BioLegend). The anti-CAR19 idiotype for surface expression of CAR19 was provided by Novartis. Cells were washed with PBS, incubated with LIVE/DEAD fixable violet (Molecular Probes) for 15 min and resuspended in fluorescence activated cell sorting buffer consisting of PBS, 1% BSA and 5 mM EDTA. The cells were then incubated with antibodies for 1 h at 4 °C. Positively stained cells were differentiated from the background using fluorescence-minus-one controls. A representative gating strategy to identify T-cell subsets is shown in Supplementary Fig. 5 . Flow cytometry was performed on a BD LSR Fortessa system. Analysis was performed using the FlowJo software (Tree Star Inc. version 10.1).
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