Cytokine analysis was carried out on the supernatants extracted from the cultures by ELISA using different ELISA kits according to the manufacturer’s instructions. The human cytokine IL-2 (ELH-IL-2, RayBio), INF-γ (ELH-IFNg, RayBio), TNF-α (ELH-TNFα, RayBio), GzmB (ELH-GZMB, RayBio), TNF-β (EK17004, SABbiotech) and The Perforin 1 (EK16250, SABbiotech) ELISA kits were used. The results were read on a multifunctional microplate reader (Tecan, Infinite M1000 PRO).
Elh tnfα
Manufactured by RayBiotech
Sourced in United States
The ELH-TNFα is a laboratory equipment designed for the detection and quantification of tumor necrosis factor alpha (TNF-α) in biological samples. It functions as an enzyme-linked immunosorbent assay (ELISA) kit, providing a reliable and sensitive method for measuring TNF-α levels.
Automatically generated - may contain errors
Lab products found in correlation
Infinite m1000 pro, by Tecan (1 mentions)
Sta 310, by Cell Biolabs (1 mentions)
Infinite m200 pro, by Tecan (1 mentions)
Anti mcl 1, by BD (1 mentions)
Anti actin, by Merck Group (1 mentions)
Anti caspase 8, by Santa Cruz Biotechnology (1 mentions)
Anti tubulin, by Merck Group (1 mentions)
Anti flag, by Merck Group (1 mentions)
Anti caspase 8, by Cell Signaling Technology (1 mentions)
Anti caspase 9, by Cell Signaling Technology (1 mentions)
Anti caspase 3, by Cell Signaling Technology (1 mentions)
Anti parp, by Cell Signaling Technology (1 mentions)
L6529, by Merck Group (1 mentions)
Elh timp2, by RayBiotech (1 mentions)
4 protocols using elh tnfα
1
Cytokine Analysis by ELISA
2
Quantifying Protein and Oxidative Markers
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The concentration of selected proteins was measured using a commercially available ELISA kit for INFγ (EHIFNG; Thermo Fisher Scientific, Waltham, United States), sTNFα (ELH-TNFα; RayBiotech Life, Atlanta, United States), and iNOS/NOS2 (E-EL-H0753; Elabscience, Houston, United States). ELISA approach was also utilized to quantify protein carbonyls (STA-310; Cell Biolabs, San Diego, United States) and oxLDL (EH0943; FineTest, Hubei, China). All assays were prepared according to the manufacturer’s instructions and measured using an Infinite M200 PRO multimode reader (TECAN, Männedorf, Switzerland).
3
Apoptosis Pathway Protein Assays
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Z-VAD was purchased from Calbiochem. The antibodies used were as follows: anti-caspase-3 (Cell signaling, 9662), anti-caspase-8 (Cell signaling, 9746), anti-caspase-8 (Santa Cruz Biotech, SC-6136), anti-caspase-9 (Cell signaling, 9508), anti-TRADD (Cell signaling, 3684), anti-actin (Sigma, A2066), anti-Flag (Sigma, F-3165), anti-tubulin (Sigma, T7816), anti-PARP (Cell signaling, 9542), anti-Mcl1 (BD, 554103), anti-TL1A (Enzo, ALX-804-859-C100), and anti-TL1A (PEPROTECH, 500-P240). Recombinant proteins: FasL (R&D, 126-FL/CF), TNFα24 (link) and TRAIL25 (link). ELISA kits: TL1A (ENZO Life Science, APO-54N-027), TNF-alpha (RayBiotech, ELH-TNFα), FasL (RayBiotech, ELH-FASL), and TRAIL (RayBiotech, ELH-TRAIL).
4
Cortisol Modulation of THP-1 Cells
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THP-1 cells were seeded at 5 × 104 cells per well in 24-well culture plates for 24 h. After incubation with starvation medium (0.5% FBS) for 24 h, cells were intervened with either human cortisol (H-CORT., 10− 9 to 10− 5 mol/L, Sigma-Aldrich, HA-8880) or PBS for 48 h. Positive control group was intervened with lipopolysaccharides (LPS, 100 ng/ml, Sigma-Aldrich, L6529). Supernatant was frozen at − 80 °C until being used for matrix metalloprotein-2 (MMP-2, Raybiotech, ELH-MMP2), tissue inhibitor of metalloproteinase-2 (TIMP-2, Raybiotech, ELH-TIMP2) and tumor necrosis factor-α (TNF-α, Raybiotech, ELH-TNFα) detection.
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