Anti gapdh d4c6r

The following primary antibodies were used in this study: anti-H3K9me3 (Abcam, cat# ab8898; IF 1:1000, WB 1:2000), anti-H3K9me2 (Active Motif, cat# 39239, IF 1:1000), anti-Lamin A/C (Santa Cruz, cat# sc-376248; IF 1:500), anti-Lamin B1 (Abcam, cat# ab16048; IF 1:1000), anti-GFP (Abcam, cat# ab290; WB 1:1000, IP 1:200), Normal Rabbit IgG (Cell Signaling, cat# 2729; IP 1:200), anti-HP1α (Abcam, cat# 77256; IF, WB 1:1000), anti-HP1β (Abcam, cat# ab10478; IF, WB 1:1000), anti-HP1γ (Santa Cruz, cat# sc-398562; IF 1:500, WB 1:1000), anti-PRR14 (Proteintech, cat# 22819-1-AP; WB 1:1000), anti-β-Actin (8H10D10) (Cell Signaling, cat# 3700S; WB 1:5000), anti-Histone H3 (Abcam, cat# ab1791; WB 1:10,000), and anti-GAPDH (D4C6R) (Cell Signaling, cat# 51332; WB 1:1000).
The following secondary antibodies from ThermoFisher Scientific/Invitrogen were used: Donkey anti-Rabbit Alexa Fluor 568 (cat# A10042, IF 1:1000), Donkey anti-Mouse Alexa Fluor 488 (cat# A10037, IF 1:1000), Donkey anti-Rabbit Alexa Fluor 647 (cat# A31573, IF 1:1000), Donkey anti-Mouse Alexa Fluor 647 (cat# A31571, IF 1:1000); and HRP-linked anti-Rabbit IgG and HRP-linked anti-Mouse IgG (Cell Signaling, cat# 7074 and 7076; WB 1:5,000). IF – immunofluorescence, WB – Western Blot, IP – immunoprecipitation.

Sub-confluent cells were lysed in Laemmli buffer (LB) and 45 µg of total proteins were subjected to SDS-PAGE and western blotting following standard methods. Protein detection was performed by using the following primary antibodies, diluted as indicated: anti-human MET (3D4, 1:3000, Invitrogen Corp., Camarillo, CA), anti-pMET Tyr^1234/1235 (D26, 1:1000), anti-JAK1 (6G4, 1:1000), anti-pJAK1 Tyr^1022/1023 (1:1000), anti-JAK2 (D2E12, 1:1000), anti-pJAK2 Tyr^1007/1008 (1:1000) anti-STAT1 (1:1000), anti-pSTAT1 Tyr⁷⁰¹ (58D6, 1:1000), anti-PD-L1 (E1L3N, 1:1000), anti-GAPDH (D4C6R, 1:1000) (all from Cell Signaling Technology, Beverly, MA), anti-IFNGR1 (EPR7866, 1:1000, Abcam, Cambridge, UK) and anti-Vinculin (hVIN-1, 1:1000, Sigma Life Sciences). Secondary HRP-conjugated goat anti-mouse IgG (1:20,000) or anti-rabbit IgG (1:20,000) (from Jackson ImmunoResearch, Cambridge, UK) and ECL System (Promega, Madison, WI) were used for protein detection.

Primary antibodies for western blotting and immunofluorescence were anti-AKT (#9272; Cell Signaling Technology, Danvers, MA), anti-αSMA (1A4, Abcam), anti-phospho-AKT (Ser473; D9E, Cell Signaling Technology), anti-phospho-YAP (Ser127; D9W2I, Cell Signaling Technology), anti-YAP (D8H1X, Cell Signaling Technology), anti-TAZ (1M19, Sigma-Aldrich), and anti-GAPDH (D4C6R, Cell Signaling Technology). Primary antibody concentrations used were as recommended for the assay by the manufacturer. Secondary antibodies for western blotting were mouse anti-rabbit (sc-2357, Santa Cruz Biotechnology, Dallas, TX) and donkey anti-mouse (715-035-151, Jackson ImmunoResearch Laboratories, West Grove, PA) and used at 1:2000 dilution. Secondary antibody for immunofluorescence was Alexa Fluor 546-conjugated goat anti-mouse (A11030, Invitrogen, Thermo Fisher Scientific) and used at 1:500 dilution. Antibodies used for protein concentration measurements were from pro-collagen 1α1 DuoSet ELISA (DY6220-05, R&D Systems) and fibronectin DuoSet ELISA (DY1918-05, R&D Systems) staining kits.