Abscess drainage fluid and surgically obtained liver specimens were sent to the bacteriology laboratory for Gram-staining and immediate inoculation onto Columbia blood agar (incubated in a CO2-enriched atmosphere), chocolate agar (incubated in a CO2-enriched atmosphere), CDC anaerobe 5% sheep blood agar (incubated under anaerobic conditions), and brain–heart infusion broth. The solid media and brain–heart infusion broth were incubated for 1 and 3 weeks, respectively, at 37°C. Blood cultures were processed by using the BD BACTECTM FX Blood Culture System (Becton Dickinson).
Bactec fx blood culture system
Manufactured by BD
Sourced in United States, Germany
The BACTEC FX blood culture system is a automated laboratory instrument designed to detect the presence of microorganisms in blood samples. It utilizes a continuous monitoring process to identify the growth of bacteria or fungi in the samples. The system provides a standardized, efficient, and consistent method for blood culture testing.
Automatically generated - may contain errors
Lab products found in correlation
Bactec plus aerobic f, by BD (4 mentions)
Columbia blood agar, by BD (2 mentions)
Biotyper library v4, by Bruker (1 mentions)
Vitek 2 automated system, by bioMérieux (1 mentions)
Maldi microflex lt, by Bruker (1 mentions)
Phoenix automated microbiology system, by BD (1 mentions)
Brain heart infusion broth, by ITW Reagents (1 mentions)
Vitek ms, by bioMérieux (1 mentions)
Vitek 2 system, by bioMérieux (1 mentions)
Bact alert blood culture system, by bioMérieux (1 mentions)
Bact alert culture media, by bioMérieux (1 mentions)
Bact alert 3d incubator, by bioMérieux (1 mentions)
Bactec plus aerobic f medium, by BD (1 mentions)
Bact alert virtuo blood culture system, by bioMérieux (1 mentions)
Vitek 2 compact system, by bioMérieux (1 mentions)
Microscan walkaway plus, by Beckman Coulter (1 mentions)
Bactec lytic 10 anaerobic f, by BD (1 mentions)
Vitek 2, by bioMérieux (1 mentions)
Bactec plus anaerobic f, by BD (1 mentions)
Biotyper system, by Bruker (1 mentions)
26 protocols using bactec fx blood culture system
1
Microbial Profiling of Abscess and Liver Samples
2
Blood Culture Processing and Antimicrobial Susceptibility Testing
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At the Institute of Medical Microbiology, UHM, pairs of aerobic/anaerobic blood culture bottles (BD BACTEC TM plus Aerobic/F and BD BACTEC TM Plus Anaerobic/F, BD, Heidelberg, Germany) were incubated for five to seven days using an automated blood culture system (BD BactecTM FX Blood Culture System, Becton Dickinson, Heidelberg, Germany). Positive blood cultures were processed in accordance with German national guidelines [24 ]. First, positive blood cultures were microscopically examined after gram staining. Next, two drops of blood culture broth were spread on Columbia blood agar (Becton Dickinson, Heidelberg, Germany) and incubated at 5% CO2 and 36 ± 1 °C. Species were identified with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) (MALDI Microflex® LT, Bruker, Bremen, Germany) using the reference Biotyper library v4.1 (Bruker Daltonik, Bremen, Germany). Antimicrobial susceptibility testing was performed using a Vitek2 automated system (bioMérieux, Marcy l′Étoile, France) applying EUCAST clinical breakpoints at the year of isolation, version 10.0 or 11.0, respectively. As a second step, linezolid resistance was confirmed using gradient tests (bioMérieux, Marcy l’Étoile, France) following EUCAST recommendations at the Institute of Hygiene, UHM. Results were interpreted according to EUCAST clinical breakpoints version 12.0.
3
Isolation and Detection of Listeria monocytogenes
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Listeria monocytogenes were isolated from blood cultures using the BACTEC FX blood culture system (BD, New Jersey, United States). Samples from other common sterile localizations were cultured on Columbia Agar with 5% Sheep Blood (agar COS, BD) and incubated at 35°C. Investigation of L. monocytogenes in feces was performed using Listeria selective-chromogenic agar (as per Ottaviani and Agosti) (RPD, Barcelona, Spain).
4
Conventional Blood Culture Procedure
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The detailed procedure of the conventional method is shown in Fig. 2 . The dialysate samples (10 mL) were inoculated into aerobic and anaerobic blood culture bottles and incubated directly in the BACTEC FX blood culture system (BD), with continuous fluorescent monitoring. This culture method served as the reference method in the study. When bacterial signals were detected, a Gram staining study was performed, and the samples were streaked onto different agar plates, such as EMB/BP and Chocolate agar (BD) for different cultures [25 (link)]. A Phoenix Automated Microbiology System, combined with MIC/ID antimicrobial susceptibility kits (BD), was used for microbial identification and antibiotic susceptibility testing. The study was performed following the manufacturer’s recommendations [25 (link)]. Patients were taken off gentamicin if the culture report showed Gram-positive bacterial. If the culture results showed methicillin-resistant staphylococcus, the vancomycin antibiotic was administered to the relevant patient.
5
Evaluation of Rapid Identification of Antibiotic-Resistant Pathogens
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The blood cultures were incubated in the BD Bactec FX blood culture system (Becton, Dickinson, Franklin Lakes, NJ). A total of 114 BD Bactec Peds Plus/F bottles (Becton, Dickinson, Franklin Lakes, NJ) were evaluated according to the standard workflow developed in our laboratory in compliance with the Clinical and Laboratory Standards Institutes (CLSI) guidelines (9 ). Prior to the implementation of the test in our laboratory, 32 bottles were spiked and tested using NG-Test CTX-M MULTI kit, and another 33 different bottles were spiked and tested using NG-Test Carba 5. For spiking, known clinical isolates from previous BSI were used. After the implementation of the test in our laboratory, an additional 49 positive blood cultures were prospectively evaluated and tested simultaneously by using both ICA kits.
6
Antimicrobial Resistance in Bloodstream Infections
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Antimicrobial susceptibility was analyzed in cases in which OB was isolated as the causative microorganism from blood culture. In patients with underlying cardiac disease, the incidence of OB with some form of antimicrobial resistance was compared with that in patients without underlying cardiac disease.
Biochemical identification was performed using the BD BACTEC FX Blood Culture System (Becton Dickinson, Sparks, MD, USA), and trypticase soy agar with 5% sheep blood/chocolate agar (Becton Dickinson, Sparks, MD, USA) was used for subculture. Antimicrobial susceptibility testing for ampicillin, penicillin G, ceftriaxone, clarithromycin, azithromycin, clindamycin, levofloxacin, and vancomycin was performed according to the agar dilution method of the Clinical Laboratory Standards Institute.8
Biochemical identification was performed using the BD BACTEC FX Blood Culture System (Becton Dickinson, Sparks, MD, USA), and trypticase soy agar with 5% sheep blood/chocolate agar (Becton Dickinson, Sparks, MD, USA) was used for subculture. Antimicrobial susceptibility testing for ampicillin, penicillin G, ceftriaxone, clarithromycin, azithromycin, clindamycin, levofloxacin, and vancomycin was performed according to the agar dilution method of the Clinical Laboratory Standards Institute.8
7
Comprehensive Microbial Identification in Biological Samples
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Samples were processed within 2 h of collection. To investigate aerobic microorganisms, 100 µL of urine, blood, and BALF from each animal were cultured on 10 mL Brain-Heart Infusion broth (PanReac-AppliChem) overnight at 37 °C; 25 µL from these cultures were plated onto CLED, MacConkey, Mannitol Salt agar, and Sabouraud Dextrose Agar (all four from PanReac-AppliChem), and incubated for 24 h at 37 °C. To investigate anaerobic and anaerobic bacteria, 3 mL of blood were inoculated on 10 mL of BD BACTEC Lytic Anaerobic (Becton Dickinson) and incubated in BD BACTEC FX blood culture system (Becton Dickinson) during 5 days. If any sign of growth was detected, a subculture was made on Blood Chocolate and McConkey agar (both of them from Beckton Dickinson), and plates were incubated for 48 h at 37 °C in 5% CO2 atmosphere and on Brucella Blood Agar with Hemin and Vitamin K1 (Beckton Dickinson) incubated for 4 days at 37 °C in anaerobiosis. Bacterial species were identified using colony morphology and Gram stain. We used MALDI-TOF MS (Vitek®MS, Biomerieux) for advanced identification.
8
Bacterial Identification and Antibiotic Susceptibility
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Blood culture was performed using the BD BACTEC™ FX blood culture system (Becton Dickinson, MA, USA). Bacterial identification and antimicrobial susceptibility tests were performed using the Vitek 2 system (bioMérieux, Marcy L'Etoile, France) following the manufacturer's recommendation. Result of the susceptibility test was interpreted based on the Clinical and Laboratory Standards Institute guidelines (CLSI) [13 14 15 ], and all results were interpreted in accordance with the changed CLSI guidelines. Intermediate susceptibility was defined being resistant.
9
Comparative Blood Culture Methodology
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The BacT/Alert culture media (aerobic, anaerobic, and pediatric) and BacT/Alert 3D incubator (bioMérieux, Durham, NC, USA) according to the manufacturer’s instructions were used during the whole study period for BC in Stockholm County. In the Skåne County, the same BacT/Alert blood culture system (bioMérieux, Durham, NC, USA) was used from 2012 to late 2014, and it was replaced by the BACTEC FX blood culture system (Becton Dickinson, Franklin Lakes, NJ, USA) using the BD BACTEC culture media (Plus Aerobic/F, Lytic/10 Anaerobic and Peds Plus/F) in December 2014, and it was used for the remaining period of the study.
10
Detecting E. coli Growth in Blood Cultures
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We used 123 non-duplicate clinical isolates of E. coli collected in the University Hospital of Besançon (France) between 2011 and 2016 and stored at the Centre de Ressources Biologiques Filière Microbiologique, Besançon (CRB-FMB, Biobanque BB-0033-00090). Table 1 details the susceptibility of E. coli isolates to AMX and CTX. We seeded blood-containing vials (BD BACTEC plus Aerobic/F medium, Becton Dickinson, Sparks, USA) with E. coli isolates for a final load of 106 CFU. Vials were incubated at 35°C in an automated blood culture system (BACTEC FX Blood Culture System, Becton Dickinson) until its detection.
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