Anti p38α mapk

Manufactured by Santa Cruz Biotechnology

Anti-p38α MAPK is a laboratory reagent that targets the p38α isoform of the mitogen-activated protein kinase (MAPK) family. It is used in research applications to study the role and function of p38α MAPK in various biological processes.

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Lab products found in correlation

Anti synaptobrevin, by Synaptic Systems (1 mentions) Immobilon western, by Merck Group (1 mentions) Phosphatase inhibitors cocktail, by Roche (1 mentions) Anti actin, by Merck Group (1 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Protease inhibitor cocktail, by Roche (1 mentions) Sc 584, by Santa Cruz Biotechnology (1 mentions) Anti p p38 mapk, by Cell Signaling Technology (1 mentions) Sc 271120, by Santa Cruz Biotechnology (1 mentions) Sc 16240, by Santa Cruz Biotechnology (1 mentions) Nbp1 45243, by Novus Biologicals (1 mentions) Anti c ebpβ, by Thermo Fisher Scientific (1 mentions) Nb100 2220, by Novus Biologicals (1 mentions) Ma1 827, by Thermo Fisher Scientific (1 mentions) Mab4470, by R&D Systems (1 mentions) Anti tlr4, by Santa Cruz Biotechnology (1 mentions) Anti p38 mapk, by Cell Signaling Technology (1 mentions) Anti p300, by Santa Cruz Biotechnology (1 mentions)

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Anti-p38α (5 citations)

2 protocols using anti p38α mapk

Western Blot Analysis of Hippocampal Proteins

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Hippocampal extracts for Western blot analysis were prepared in lysis buffer containing 10 mM HEPES pH 7.4, 150 mM NaCl, 10 mM EDTA, 1% Triton, phosphatase inhibitors cocktail (Roche), and a protease inhibitor cocktail (Roche). Samples were homogenized at 4 °C, and protein content was determined by the Pierce^® BCA Protein Assay kit (Thermo Scientific). Total protein (20–40 µg) was processed on 8% SDS–PAGE and transferred to a PVDF membrane (Immoblot-P Millipore). After blocking with 5% non-fat dried milk, blots were incubated at 4 °C overnight with primary antibodies anti-p38α MAPK (Santacruz, sc-535), anti-p38β MAPK (Thermo Scientific, 33-8700), anti-actin (Millipore, MAB1501R), anti-VAMP3 Thermo Scientific, PA1-767A), and anti-synaptobrevin (Synaptic Systems, clone 91-1). Corresponding secondary antibodies (peroxidase-conjugated anti-rabbit or anti-mouse; Jackson ImmunoResearch 711-035-152, Sigma I5381) were incubated for 1 h. Detection was carried out by chemiluminescence (Immobilon Western, Millipore) using ImageQuant™ LAS 4000 mini biomolecular imager.

Navarrete M., Cuartero M.I., Palenzuela R., Draffin J.E., Konomi A., Serra I., Colié S., Castaño-Castaño S., Hasan M.T., Nebreda Á.R, & Esteban J.A. (2019). Astrocytic p38α MAPK drives NMDA receptor-dependent long-term depression and modulates long-term memory. Nature Communications, 10, 2968.

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Antibody Validation for Protein Analysis

Cited 1 time

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All procedures were adhered to our previous publication (12 (link)). The following primary antibodies were used: anti-p300 (1:500, sc584, Santa Cruz), anti-C/EBPβ (1:1000, MA1–827, Thermo Fisher), anti-pT188-C/EBPβ (1:1000, 3084, Cell Signaling Technology), anti-TLR4 (1:500, sc16240, Santa Cruz), anti-p38α MAPK (1:500, sc271120, Santa Cruz), anti-p38β MAPK (1:500, 2339, Cell Signaling Technology), anti-p38 MAPK (1:1000, 9212, Cell Signaling Technology), anti-p-p38 MAPK (1:1000, 4511, Cell Signaling Technology), anti-MAFbx (1:1000, AP2041, ECM Bioscience), anti-UBR2 (1:500, NBP1–45243, Novus Biologicals), anti-LC3 (1:2000, NB100–2220, Novus Biologicals) and anti-MHC (1:1000, MAB4470, R&D Systems). Antibody against acetylated Lys-39 of C/EBPβ (1:2000) was generated as previously described (12 (link)). Antibody against phosphorylated Ser-12 of p300 (1:1500) were generated by Pocono Rabbit Farm & Laboratory (Canadensis, PA) from rabbit using the peptide PGPPS(P)AKRPKLSSPAC. The specificity of the antibody was validated using mutated p300 with point mutantions (Figure 1A). Data were normalized to α-Tubulin (Development Studies Hybridoma Bank at the University of Iowa, Iowa City, IA).

Sin T.K., Zhang G., Zhang Z., Zhu J.Z., Zuo Y., Frost J.A., Li M, & Li Y.P. (2020). Cancer-induced muscle wasting requires p38β MAPK-mediated activation of p300. Cancer research, 81(4), 885-897.

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