Horgc120pg05

We next identified SOAT protein levels in different types of cancer. SOAT1 expression was assessed through immunohistochemistry via a multiple-cancer tissue microarray (HOrgC120PG05, Shanghai Outdo Biotech Co., Ltd., Shanghai, China). Rabbit anti-SOAT1 polyclonal antibody was obtained from Ab-Mart (Shanghai, China) and used at 1:100 dilution. A total of 120 paraffin-embedded organic tissues were obtained for analysis, including 9 normal tissue samples and samples from 10 types of cancer and their paired paracancerous tissue. Eight breast cancer tissues were also obtained but lacked paracancerous or normal tissue controls; thus, these data were excluded.

Human multiple organ tissue arrays (Cat# HOrgC110PT01 and HOrgC120PG05, Shanghai Outdo Biotech Co., Ltd., Shanghai, China) were used to test the protein expression of PNPO by immunohistochemistry. The study of the human subject was approved by the Ethics Committee of Shanghai Outdo Biotech Co., Ltd. A total of 230 paraffin-embedded tissue samples were collected, and of these, 2 samples were either invalid or missing. All the other 228 samples were valid, including 121 malignant tumor tissues, 89 adjacent tissues of cancer, and 18 normal tissues (Supplementary Table S1). The anti-PNPO antibody (1:2000 dilution, #15552-1-AP) was obtained from ProteinTech Group (Chicago, IL, United States).