Fibronectin (FN) from human plasma (Sigma-Aldrich, St. Louis, MO, USA) was adsorbed onto the PVDF samples. The biomaterials were immersed in a FN solution of 20 μg/mL for 1 h under constant shaking. After protein adsorption, the samples were rinsed in saline solution to eliminate the non-adsorbed protein.
Fibronectin fn from human plasma
Manufactured by Merck Group
Sourced in United States
Fibronectin (FN) from human plasma is a glycoprotein that plays a role in cell adhesion, growth, migration, and differentiation. It is a component of the extracellular matrix and is involved in various biological processes. The product is intended for research use only.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using fibronectin fn from human plasma
1
Fibronectin Adsorption on PVDF
2
Cardiac Cell Co-Culture Protocol
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All of the reagents were used without further purification. Fibronectin (FN) from human plasma, gelatin (G), Dulbecco’s modified eagle medium (DMEM), fetal bovine serum and the cell culture insert with a 0.4 or 3 µm pore membrane was purchased from Sigma-Aldrich (MO, USA), Wako Pure Chemical Industries (Osaka, Japan), Nacalai Tesque (Kyoto, Japan), Life Technologies (CA, USA) and Corning (NY, USA), respectively. Normal human cardiac fibroblasts (NHCFs), normal human cardiac microvascular endothelial cells (HCMVECs), cardiac fibroblast growth medium (FGM-3) and endothelial growth medium (EGM-2 MV) were further purchased from Lonza (NJ, USA).
3
Fibronectin Adsorption on PVDF Films
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Fibronectin (FN) from human plasma (Sigma-Aldrich, St. Louis, MO, USA) was adsorbed onto the PVDF samples. The films were immersed in a 20 μg/mL FN solution for 1 h under constant shaking. After protein adsorption, the samples were rinsed in saline solution to eliminate the non-adsorbed protein.
4
Layered Fibronectin-Gelatin Coating of Cells
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Fibronectin (FN) from human plasma (MW = 4.6 × 105 Da) was purchased from Sigma‐Aldrich (St. Louis, MO, USA), and gelatin (G) (MW =1.0 × 105 Da) was purchased from Wako Pure Chemical Industries (Osaka, Japan). All chemicals were used without purification. For LbL filtration, 2.5 mL of a solution containing 0.2 mg/mL FN and G in 50 mM Tris–HCl (pH =7.4) were added to three wells of a 6‐well plate. NHDFs dissociated with 0.1% trypsin were resuspended in 500 µL of 0.2 mg/mL FN and G/Tris–HCl solution and transferred to a 6‐well plate culture insert with a membrane pore size of 0.4 µm (Corning, Inc., Corning, NY, USA). The insert was placed in the well containing the FN and G/Tris–HCl solution and alternately incubated for 1 min in a shaking incubator (SI‐300; As One Co., Osaka, Japan) and washed. To collect the coated cells at each step, the insert was transferred to an empty well and shaken horizontally at 1.1 g to filter the cell suspension.2
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