Sin 1

Manufactured by Bio-Techne

Sourced in United States

SIN-1 is a laboratory reagent used in biochemical research. It is a peroxynitrite donor that can be used to study the effects of peroxynitrite in cellular and molecular biology experiments.

Automatically generated - may contain errors

Lab products found in correlation

L name, by Cayman Chemical (1 mentions) Boldine, by Merck Group (1 mentions) Water jacketed co2 incubator, by Thermo Fisher Scientific (1 mentions) Bapta, by Bio-Techne (1 mentions) L nio, by Bio-Techne (1 mentions) Thrombin, by Merck Group (1 mentions) Daf fm diacetate, by Thermo Fisher Scientific (1 mentions)

2 protocols using sin 1

Retinal Explant Culture in Diabetic Conditions

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A total of 97 retinal explants obtained from P9 wild-type mice from 50 wild-type animals were cultured as previously described [32 (link),33 (link)] and divided among the experiments. Briefly, the retina was placed with the pigment epithelium facing down on cell culture inserts (Millicell, PIHA03050, Merck, Darmstadt, Germany) with 1 mL of culture containing 10% of fetal bovine serum (FBS), which was replaced every two days. The cultures were incubated at 37 °C in a 5% CO₂ water-jacketed incubator (Thermo Scientific, Waltham, MA, USA). Once retinal explants were cultured, an adaptation period of two days was performed before the start of the different treatments. Moderate glucose concentration (15 mM) was used as a control, and high glucose (30 mM) was used to simulate diabetic conditions. The reagents used in this study were boldine (100 µM) (Cat# B3916-5, Sigma Aldrich, St. Louis, MO, USA), L-NAME (1 mM) (Cat# 80210, Cayman, Ann Harbor, MI, USA), and SIN-1 (10 µM) (Cat# 0756, Tocris Bioscience, Bristol, UK).

Calbiague García V., Cadiz B., Herrera P., Díaz A, & Schmachtenberg O. (2023). Evaluation of Photobiomodulation and Boldine as Alternative Treatment Options in Two Diabetic Retinopathy Models. International Journal of Molecular Sciences, 24(9), 7918.

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Nitric Oxide Signaling Pathway Analysis

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Thrombin was purchase from Sigma-Aldrich (Cat#: T4393, St. Louis, MO). L-NIO [N⁵-(1-Iminoethyl)-L-ornithine dihydrochloride], SIN-1 (3-morpholinosydnonimine chloride), and BAPTA [1,2-Bis(2-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid] were purchase from Tocris (Cat#: 0546, 0756, and 2786, respectively, Minneapolis, MN). RhoA inhibitor I (highly purified C3 transferase) was purchased from Cytoskeleton Inc. (Cat# CT04). FeTPPS [5,10,15,20-Tetrakis(4-sulfonatophenyl)porphyrinato Iron (III), Cl] and DETA-NO (diethylamine NONOate/AM) was purchase from Millipore-Calbiochem (Cat#: 341492 and 292505, respectively, Billerica, MA). DAF-FM diacetate (4-amino-5-methylamino-2’,7’-difluorofluorescein diacetate) was purchased from Thermofisher Scientific (Cat# D-23842, Waltham, MA) S-nitrosoglutathione (GSNO) was purchase from World precision instruments (Cat#: GSNO-100, Sarasota, FL). The effective concentration of the GSNO was calculated from the optical absorbance at 338 nm and the reported molar extinction coefficients as described previously [34 (link)].

Choi S., Saxena N., Dhammu T., Khan M., Singh A.K., Singh I, & Won J. (2018). Regulation of endothelial barrier integrity by redox-dependent nitric oxide signaling: Implication in traumatic and inflammatory brain injuries. Nitric oxide : biology and chemistry, 83, 51-64.

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