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Mass elisa kits

Manufactured by Echelon Biosciences
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Mass ELISA kits are laboratory equipment designed to perform enzyme-linked immunosorbent assays (ELISA) on multiple samples simultaneously. These kits provide a standardized and efficient method for the quantitative detection and measurement of specific analytes, such as proteins, hormones, or other biomolecules, in biological samples.

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Lab products found in correlation

Spectramax i3 plate, by Molecular Devices (2 mentions) Prism 7, by GraphPad (2 mentions) Infinite 200, by Tecan (1 mentions) Synergy 2 multi detection microplate reader, by Agilent Technologies (1 mentions) Spectramax i3, by Molecular Devices (1 mentions)

5 protocols using mass elisa kits

1

Quantifying Phosphoinositide Levels

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1 × 106 B cells were collected per time point. Samples were processed per the manufacturers’ instructions included in the Echelon Biosciences Mass ELISA kits for PtdIns(4,5)P2 (K-4500), PtdIns(3,4,5)P3 (K-2500s) and PtdIns(3,4)P2 (K-3800). The mass ELISA assays were measured at 450 nM on a Molecular Devices SpectraMax i3 plate reader. The standard curve was fit assuming a sigmoidal dose-response with variable slope and the amount of phosphatidylinositol in each sample was extrapolated in the GraphPad Prism 7 software package.
Luo W., Hawse W., Conter L., Trivedi N., Weisel F., Wikenheiser D., Cattley R.T, & Shlomchik M.J. (2019). The AKT kinase signaling network is rewired by PTEN to control proximal BCR signaling in germinal center B cells. Nature immunology, 20(6), 736-746.
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2

Quantifying Phosphoinositide Levels

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1 × 106 B cells were collected per time point. Samples were processed per the manufacturers’ instructions included in the Echelon Biosciences Mass ELISA kits for PtdIns(4,5)P2 (K-4500), PtdIns(3,4,5)P3 (K-2500s) and PtdIns(3,4)P2 (K-3800). The mass ELISA assays were measured at 450 nM on a Molecular Devices SpectraMax i3 plate reader. The standard curve was fit assuming a sigmoidal dose-response with variable slope and the amount of phosphatidylinositol in each sample was extrapolated in the GraphPad Prism 7 software package.
Luo W., Hawse W., Conter L., Trivedi N., Weisel F., Wikenheiser D., Cattley R.T, & Shlomchik M.J. (2019). The AKT kinase signaling network is rewired by PTEN to control proximal BCR signaling in germinal center B cells. Nature immunology, 20(6), 736-746.
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3

Quantifying Cellular Phosphoinositides

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The PI(3)P (#K-3300) and PI(3,4,5)P3 (#K-2500) Mass ELISA Kits (both from Echelon Biosciences, Salt Lake City, UT) were used as recommended by the manufacturer. Acidic lipids were extracted from 5*106 cells. OD values from serial dilutions of lipids (pure, 1:2, 1:4 and 1:8) were measured using a microplate reader (Infinite 200, Tecan) and calculated as pmol per 106 cells from the standard curve using non-linear curve fitting software (Graphpad Prism 4.0).
Weidner P., Söhn M., Gutting T., Friedrich T., Gaiser T., Magdeburg J., Kienle P., Ruh H., Hopf C., Behrens H.M., Röcken C., Hanoch T., Seger R., Ebert M.P, & Burgermeister E. (2016). Myotubularin-related protein 7 inhibits insulin signaling in colorectal cancer. Oncotarget, 7(31), 50490-50506.
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4

Quantitative Analysis of Cellular Lipids

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Cellular PI(3,4,5)P3, PI(3,4)P2 and PI(3)P were respectively extracted and quantitated using PI(3,4,5)P3, PI(3,4)P2 and PI(3)P Mass ELISA kits from Echelon Biosciences (Salt Lake City, UT, USA) according to the manufacturer's instruction. The results were recorded and analyzed using Synergy 2 multi-detection microplate reader (BioTek, Winooski, VT, USA).
Guo S.T., Chi M.N., Yang R.H., Guo X.Y., Zan L.K., Wang C.Y., Xi Y.F., Jin L., Croft A., Tseng H.Y., Yan X.G., Farrelly M., Wang F.H., Lai F., Wang J.F., Li Y.P., Ackland S., Scott R., Agoulnik I.U., Hondermarck H., Thorne R.F., Liu T., Zhang X.D, & Jiang C.C. (2015). INPP4B is an oncogenic regulator in human colon cancer. Oncogene, 35(23), 3049-3061.
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5

Quantifying Murine T Cell Lipid Signaling

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Murine CD4+ T cells isolated by negative selection were activated. Pellets from 2 million cells were washed with ice cold 0.5 M TCA and treated with 750 μl of MeOH: CHCl3: 12N HCl (80:40:1), vortexing for 30 min and centrifuging for 10 min at 3000 RPM to remove neutral lipids. The supernatant was treated with 250 μl of CHCl3 and 450 μl of 0.1 N HCl. The organic phase was dried under a stream of nitrogen gas and reconstituted in PBS. Mass ELISA kits from Echelon Biosciences were used to measure PtdIns(3,4,5)P3 and PtdIns(4,5)P2 using the manufacturer’s protocol using a Molecular Devices SpectraMax i3 plate reader.
Prado D.S., Cattley R.T., Shipman C.W., Happe C., Lee M., Boggess W.C., MacDonald M.L, & Hawse W.F. (2021). Synergistic and additive interactions between receptor signaling networks drive the regulatory T cell versus T helper 17 cell fate choice. The Journal of Biological Chemistry, 297(6), 101330.
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