Plko 1 control

An expression vector for human PTK7 with a C-terminal Flag tag (pcDNA3-PTK7-FLAG) and a knockdown vector for human PTK7 (pLKO.1-shRNA-PTK7-6434) and an empty knockdown vector (pLKO.1-control) (Sigma-Aldrich, St. Louis, MO, USA) were described previously^{4 (link),25 (link)}.

Expression vectors pcDNA3.1-TIMP-1 [42 (link)] and pcDNA3.1-TIMP-2 [43 (link)] encoding human TIMP-1 and TIMP-2, respectively, and pcDNA3-hPTK7-FLAG [17 (link)] encoding human PTK7 with a C-terminal Flag tag were described previously. Expression vector encoding K297R/Y529F dominant-negative mouse Src (pLNCX-mSrc [K297R/Y529F]) was generated by site-directed mutagenesis using pLNCX-mSrc, which was a generous gift from Professor E.-S. Oh (Ewha Womans University, Korea), as a template and primer pairs listed in Supplementary Table S1. Expression vector encoding HA-tagged K179M kinase-deficient AKT (pCMV6-HA-Akt-KD [K179M]) was a kind gift of Professor K.-Y. Choi (Yonsei University, Korea). Constructs pLKO.1-shRNA-PTK7–6433 and −6434 for human PTK7 knockdown vectors and pLKO.1-control (Sigma-Aldrich) were described previously [8 (link)]. Constructs LentiCRISPRv2-sgRNA-PTK7-F369 and –F849 for human PTK7 knockout vectors were generated by cloning of oligonucleotide sequences listed in Supplementary Table S2 into LentiCRISPRv2 vector (Addgene, Cambridge, MA, USA).