Accuri cflow 6

Manufactured by BD

The BD Accuri C6 Flow Cytometer is a compact and easy-to-use instrument designed for routine flow cytometric analysis. It features six-color detection capabilities and is capable of analyzing a wide range of sample types, including cells, microbeads, and other suspensions.

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BD Accuri (258 citations) CFlow (20 citations) Accuri 6 (15 citations) Accuri CFlow@ (8 citations)

3 protocols using accuri cflow 6

Quantifying Apoptosis in AML Cells

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Following designated treatments (72–96 hours), cultured cell lines or patient-derived (PD) AML blast cells, were washed with 1× PBS, stained with TO-PRO-3 iodide (Cat# T3605, Life Technologies, Carlsbad, CA), and analyzed by flow cytometry on a BD Accuri CFlow-6 flow cytometer (BD Biosciences, San Jose, CA).

Mill C.P., Fiskus W., Das K., Davis J.A., Birdwell C.E., Kadia T.M., DiNardo C.D., Daver N., Takahashi K., Sasaki K., McGeehan G.M., Ruan X., Su X., Loghavi S., Kantarjian H, & Bhalla K.N. (2023). Causal linkage of presence of mutant NPM1 to efficacy of novel therapeutic agents against AML cells with mutant NPM1. Leukemia, 37(6), 1336-1348.

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Synergistic Drug Combination Screening

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Following designated treatments, cultured cell lines or PD-AML cells were washed with 1× phosphate-buffered saline, stained with TO-PRO-3 iodide (Life Technologies, Carlsbad, CA), and analyzed by flow cytometry on a BD Accuri CFlow-6 flow cytometer (BD Biosciences, San Jose, CA). We used matrix dosing of agents in combinations to allow synergy assessment utilizing the SynergyFinder V2 online web application tool (http://synergyfinder.fimm.fi/). Delta synergy scores were determined by the ZIP method.

Fiskus W., Boettcher S., Daver N., Mill C.P., Sasaki K., Birdwell C.E., Davis J.A., Takahashi K., Kadia T.M., DiNardo C.D., Jin Q., Qi Y., Su X., McGeehan G.M., Khoury J.D., Ebert B.L, & Bhalla K.N. (2022). Effective Menin inhibitor-based combinations against AML with MLL rearrangement or NPM1 mutation (NPM1c). Blood Cancer Journal, 12(1), 5.

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Cell Viability Assessment by Flow Cytometry

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Following designated treatments (72–96 h), cultured cell lines or patient-derived (PD) AML blast cells, were washed with 1× PBS, stained with TO-PRO-3 iodide (Cat# T3605, Life Technologies, Carlsbad, CA) and analyzed by flow cytometry on a BD Accuri CFlow-6 flow cytometer (BD Biosciences, San Jose, CA).

Fiskus W., Mill C.P., Birdwell C., Davis J.A., Das K., Boettcher S., Kadia T.M., DiNardo C.D., Takahashi K., Loghavi S., Soth M.J., Heffernan T., McGeehan G.M., Ruan X., Su X., Vakoc C.R., Daver N, & Bhalla K.N. (2023). Targeting of epigenetic co-dependencies enhances anti-AML efficacy of Menin inhibitor in AML with MLL1-r or mutant NPM1. Blood Cancer Journal, 13(1), 53.

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