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4 4 dithiodibutyricacid

Manufactured by Merck Group
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4,4′-Dithiodibutyricacid is a chemical compound used in laboratory settings. It serves as a core functional component in various analytical and research applications. The detailed properties and specific uses of this product require further technical information that is beyond the scope of this response.

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Lab products found in correlation

3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Trypsin edta solution, by Merck Group (2 mentions) Penicillin streptomycin solution, by Thermo Fisher Scientific (2 mentions) Triethylamine, by Merck Group (2 mentions) Rat tail collagen 1, by BD (1 mentions) N ethyl n isopropylpropan 2 amine, by Merck Group (1 mentions) N n dimethylpyridin 4 amine dmap, by Merck Group (1 mentions) Ac 500 mhz, by Bruker (1 mentions) Ebm 2 growth medium, by Lonza (1 mentions) Calcein am, by Thermo Fisher Scientific (1 mentions) Dithiothreitol (dtt), by Roche (1 mentions) 4 dimethylaminopyridine, by Merck Group (1 mentions) Trifluoroacetic acid, by Thermo Fisher Scientific (1 mentions) Fluorescein isothiocyanate dextran, by Merck Group (1 mentions) P toluenesulfonic acid monohydrate, by Merck Group (1 mentions) 3 mercaptopropionic acid, by Merck Group (1 mentions) N n dicyclohexylcarbodiimide, by Merck Group (1 mentions) Toluene, by Merck Group (1 mentions) Lactate colorimetric fluorometric assay kit, by Abcam (1 mentions)

4 protocols using 4 4 dithiodibutyricacid

1

Synthesis and Characterization of Small Molecule Inhibitors

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Abacavir,
darunavir, and nelfinavir were
provided by the NIH AIDS Reagent Program (Germantown, MD). ((1H-Benzo[d][1,2,3]triazol-1-yl)oxy)tri(pyrrolidin-1-yl)phosphonium
hexafluorophosphate (PyBOP) was bought from GenScript Corporation
(Piscataway, NJ). DTT was purchased from Roche (Indianapolis, IN).
1-(3-Dimethylaminoproyl)-3-ethylcarbodiimide hydrochloride (EDC) was
purchased fom AK Scientific (Union City, CA). 4,4′-Dithiodibutyric
acid, N,N-dimethylpyridin-4-amine
(DMAP), and N-ethyl-N-isopropylpropan-2-amine
(DIEA) were purchased from Sigma-Aldrich (St. Louis, MO). hCMEC/D3
cells were donated from Institut National de la Sante et de la Recherche
Medicale (INSERM, Paris, France). Rat tail collagen I was purchased
from BD Biosciences (San Jose, CA). EBM-2 growth medium was purchased
from Lonza (Basel, Switzerland). Calcein-AM was bought from Invitrogen
(Carlsbad, CA), All other reagents were purchased from Sigma-Aldrich
(St. Louis, MO) or Invitrogen (Carlsbad, CA) and used without further
purification. 1H, 13C, and 2D NMR spectra were
recorded with a Bruker AC 800 and Bruker AC 500 MHz. DMSO-d6 was used to prepare NMR samples.
Agrawal N., Rowe J., Lan J., Yu Q., Hrycyna C.A, & Chmielewski J. (2019). Potential Tools for Eradicating HIV Reservoirs in the Brain: Development of Trojan Horse Prodrugs for the Inhibition of P-Glycoprotein with Anti-HIV-1 Activity. Journal of Medicinal Chemistry, 63(5), 2131-2138.
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2

Functionalized PEG Hydrogel Synthesis

Cited 1 time
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Triethylamine and diethyl ether
were purchased from Merck. 1,4-Dithio-dl-threitol (DTT) (98%)
and trifluoroacetic acid were purchased from Alfa Aesar. 4-(Dimethylamino)pyridine
(DMAP, >99%), 4,4′-dithiodibutyric acid (95%), 3-mercaptopropionic
acid, N,N′-dicyclohexylcarbodiimide
(DCC), PEG (4 kDa), toluene, fluorescein isothiocyanate–dextran
(FITC-dextran, 20, 150 kDa), albumin–FITC conjugate (FITC–BSA),
and p-toluenesulfonic acid monohydrate (99%) were
purchased from Sigma-Aldrich. Four-arm PEG (10 kDa) was purchased
from Creative PEGWorks. The thiol-terminated four-arm PEG54 (link) and the furan-maleimide adduct containing alcohol55 (link) were obtained using literature procedures.
Altinbasak I., Kocak S., Sanyal R, & Sanyal A. (2022). Fast-Forming Dissolvable Redox-Responsive Hydrogels: Exploiting the Orthogonality of Thiol–Maleimide and Thiol–Disulfide Exchange Chemistry. Biomacromolecules, 23(9), 3525-3534.
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3

Synthesis and Characterization of V9302 Compound

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V9302 was synthesized and purified following a published paper[41 (link)]. 4-Vinylbenzyl chloride, 4,4-dithiodibutyric acid, triethylamine, 4-Cyano-4-(phenyl-carbonothioylthio)pentanoic acid, poly(Ethylene glycol)methyl ether methacrylate (average Mn = 950), 2,2-Azobis (isobutyronitrile)(AIBN), Dulbecco’s Modified Eagle’s Medium (DMEM), RPMI 1640 Medium, trypsin-EDTA solution, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) were purchased from Sigma-Aldrich (MO, U.S.A). 2-deoxy-D-glucose was purchased from AK Scientific Inc. (CA, U.S.A.). Antibody against ASCT2 was purchased from Cell Signaling Technology, Inc. (MA, U.S.A). SuperSignal™ West Fento Maximum Sensitivity Substrate Kit and Pierce™ RIPA buffer were purchased from Thermo Scientific (MA, U.S.A). Fetal bovine serum (FBS), penicillin-streptomycin solution and Trizol lysis reagent were purchased from Invitrogen (NY, U.S.A.). QuantiTect Reverse Transcription Kit was purchased from Qiagen (MD, U.S.A). EnzyChrom Glutamine Assay kit was purchased from BioAssay Systems (CA, USA), Glucose Colorimetric/Fluorometric Assay Kit and Lactate Colorimetric/Fluorometric Assay Kit were purchased from BioVision Inc. (CA, USA). ALT/SGPT Liqui-UV Test Kit and AST/SGOT Liqui-UV Test Kit were purchased from Stanbio Laboratory (TX, USA).
Luo Z., Xu J., Sun J., Huang H., Zhang Z., Ma W., Wan Z., Liu Y., Pardeshi A, & Li S. (2020). Co-delivery of 2-Deoxyglucose and a Glutamine Metabolism Inhibitor V9302 via a Prodrug Micellar Formulation for Synergistic Targeting of Metabolism in Cancer. Acta biomaterialia, 105, 239-252.
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4

Multifunctional Polymer-based Theranostics

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Vinylbenzyl chloride, 4,4'-Dithiodibutyric acid, oligo(ethylene glycol) methacrylate (OEG950 monomer, average Mn=950), 4-Cyano-4-(phenylcarbonothioylthio)pentanoic acid, 2, 2-Azobis(isobutyronitrile) (AIBN), trypsin-EDTA solution, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and Dulbecco's Modified Eagle's Medium (DMEM) were all bought from Sigma-Aldrich (MO, U. S. A.). AIBN was purified by recrystallization in anhydrous ethanol. 1-hydroxybenzotriazole (HOBT) and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide HCl (EDC) were purchased from GL Biochem (Shanghai, China). Diisopropylethylamine (DIPEA) was purchased from Acros Organics. Paclitaxel was purchased from AK Scientific Inc. (CA, U. S. A.). Doxorubicin hydrochloride salt (DOX·HCl) and gemcitabine (GEM) were purchased from LC Laboratories (MA, USA). Fetal bovine serum (FBS) and penicillin-streptomycin solution were purchased from Invitrogen (NY, U. S. A.). The animal-related experiments were performed in full compliance with institutional guidelines and approved by the Animal Use and Care Administrative Advisory Committee at the University of Pittsburgh. Informed consent was obtained for experimentation with patient-derived xenograft (PDX) model. And the PDX-related experiments were carried out in accordance with The Code of Ethics of the World Medical Association.
Sun J., Chen Y., Xu J., Song X., Wan Z., Du Y., Ma W., Li X., Zhang L, & Li S. (2020). High Loading of Hydrophobic and Hydrophilic Agents via Small Immunostimulatory Carrier for Enhanced Tumor Penetration and Combinational Therapy. Theranostics, 10(3), 1136-1150.
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