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Anti fibrillrin

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Anti-fibrillrin is a laboratory reagent used to detect and quantify the presence of fibrillarin, a nuclear protein involved in ribosome biogenesis and small nucleolar ribonucleoprotein (snoRNP) assembly. It can be used in various techniques, such as Western blotting, immunoprecipitation, and immunofluorescence microscopy, to study the expression and localization of fibrillarin in biological samples.

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Lab products found in correlation

Anti β actin, by Merck Group (2 mentions) Anti phospho egfr tyr1068, by Cell Signaling Technology (2 mentions) Ne per nuclear and cytoplasmic extraction reagent, by Thermo Fisher Scientific (2 mentions) Anti aid, by Thermo Fisher Scientific (2 mentions) Bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Anti phospho ser473 p akt, by Cell Signaling Technology (2 mentions) Anti total egfr, by Merck Group (2 mentions) Anti april, by Thermo Fisher Scientific (2 mentions) Anti p65, by Santa Cruz Biotechnology (2 mentions)

2 protocols using anti fibrillrin

1

Quantitative Western Blot Analysis of Immune Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
MSIE cells and intestinal epithelial cells isolated from mice were solubilized in cell lysis buffer to prepare total cellular lysates. Nuclear fractions were prepared using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific) according to the manufacturer’s instructions. The protein concentration was determined using a BCA protein assay kit (Thermo Fisher Scientific Inc.).
Cellular and nuclear lysates were mixed with Laemmli sample buffer and proteins were separated by SDS-PAGE for Western blot analysis using anti-phospho-Ser473(P)-Akt (Cell Signalling Danvers, MA), anti-phospho-Tyr1068-EGFR (Cell Signalling), anti-total EGFR (Millipore, Billerica, MA), anti-APRIL (Thermo scientific), anti-BAFF (Enzo Biochem, Inc, Farmingdale, NY), anti-AID (eBioscience), anti-p65 (Santa Cruze, Dallas, TX), anti-fibrillrin (Santa Cruze), and anti-β actin (Sigma-Aldrich) antibodies.
Wang Y., Liu L., Moore D.J., Shen X., Peek RM J.r., Acra S.A., Li H., Ren X., Polk D.B, & Yan F. (2016). A LGG-derived protein promotes IgA production through up-regulation of APRIL expression in intestinal epithelial cells. Mucosal immunology, 10(2), 373-384.
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2

Quantitative Western Blot Analysis of Immune Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
MSIE cells and intestinal epithelial cells isolated from mice were solubilized in cell lysis buffer to prepare total cellular lysates. Nuclear fractions were prepared using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific) according to the manufacturer’s instructions. The protein concentration was determined using a BCA protein assay kit (Thermo Fisher Scientific Inc.).
Cellular and nuclear lysates were mixed with Laemmli sample buffer and proteins were separated by SDS-PAGE for Western blot analysis using anti-phospho-Ser473(P)-Akt (Cell Signalling Danvers, MA), anti-phospho-Tyr1068-EGFR (Cell Signalling), anti-total EGFR (Millipore, Billerica, MA), anti-APRIL (Thermo scientific), anti-BAFF (Enzo Biochem, Inc, Farmingdale, NY), anti-AID (eBioscience), anti-p65 (Santa Cruze, Dallas, TX), anti-fibrillrin (Santa Cruze), and anti-β actin (Sigma-Aldrich) antibodies.
Wang Y., Liu L., Moore D.J., Shen X., Peek RM J.r., Acra S.A., Li H., Ren X., Polk D.B, & Yan F. (2016). A LGG-derived protein promotes IgA production through up-regulation of APRIL expression in intestinal epithelial cells. Mucosal immunology, 10(2), 373-384.
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