P. serotine seeds were obtained from Xochimilco’s market in Mexico City, Mexico. Wheat flour (Triticum spp.) and canola oil were purchased from a local food store in Monterrey, Nuevo Leon, Mexico. Whey protein concentrate (WPC, MB Pro-mix, 80%) was food grade from Marquez Bros, International, Inc.-whey division, Hanford, CA. Solvents: hexane, n-propanol, boric acid, ethanol, phosphoric acid, and hydrochloric acid were of analytical grade (J.T. Baker reagents, Azcapotzalco, Mexico City, Mexico). The reagents sodium chloride, sodium hydroxide, sodium azide, sodium carbonate, sodium tartrate and copper sulphate were purchased from Development of Chemical Specialities in Monterrey, Nuevo Leon, Mexico. Picric acid was from Acce Microbiology in Guadalupe, Nuevo Leon, Mexico, and Coomassie brilliant blue G-250 from ThermoFisher Scientific, Mexico. Sucrose, Tris(hydroxymethyl)aminomethane, SDS, Folin & Ciocalteu’s and bovine serum albumin (BSA) were from Sigma-Aldrich, Mexico.
Folin ciocalteu s
Manufactured by Merck Group
Sourced in United States
Folin-Ciocalteu's is a colorimetric reagent used for the quantitative determination of phenolic compounds. It measures the total phenolic content in a sample by analyzing the reduction of phosphomolybdic-phosphotungstic acid complexes.
Automatically generated - may contain errors
Lab products found in correlation
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (2 mentions)
Coomassie brilliant blue g 250, by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
Sucrose, by Merck Group (1 mentions)
Tris hydroxymethyl aminomethane, by Merck Group (1 mentions)
2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts), by Merck Group (1 mentions)
Absolute ethanol, by Avantor (1 mentions)
Rpmi 1640, by Merck Group (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Quercetin, by Merck Group (1 mentions)
Andrographolide, by Merck Group (1 mentions)
Clindamycin phosphate, by Merck Group (1 mentions)
Gallic acid, by Bio Basic (1 mentions)
Bismuth iii nitrate, by Merck Group (1 mentions)
Fluorescence mounting medium, by Agilent Technologies (1 mentions)
Indomethacin, by Merck Group (1 mentions)
Dihydroethidium, by Merck Group (1 mentions)
Na2co3, by Vetec (1 mentions)
7 protocols using folin ciocalteu s
1
Preparation and Characterization of P. serotine Seed Flour
2
Chemical Reagents Procurement for Scientific Investigations
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The chemicals and reagents used
for the experiments carried out for the present investigation were
purchased from Spectrochem, SRL, Acros-Organics, RANKEM, and Fisher
Scientific and used as received without further purification. ABTS,
DPPH, and Folin-Ciocalteu’s reagents were obtained from Sigma-Aldrich.
for the experiments carried out for the present investigation were
purchased from Spectrochem, SRL, Acros-Organics, RANKEM, and Fisher
Scientific and used as received without further purification. ABTS,
DPPH, and Folin-Ciocalteu’s reagents were obtained from Sigma-Aldrich.
3
Cytotoxicity Evaluation of Bioactive Compounds
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Clindamycin phosphate, Penicillin/Streptomycin (P/S), 0.25% trypsin/EDTA, Veratrine alkaloid, RPMI-1640, Fetal bovine serum (FBS), Bismuth III nitrate, atropine, quercetin, andrographolide, Folin-Ciocalteus, and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) reagents were purchased from Sigma Aldrich, USA. Other chemicals such as dimethyl sulphoxide (DMSO), bromocresol green, and gallic acid were purchased from Biobasic, Canada, while absolute ethanol was from VWR Chemicals, France.
4
Spectrophotometric Quantification of QTI
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The TPC of QTI was measured spectrophotometrically (UV–VIS spectrophotometer, XD-7500, Lovibond, Germany) at 765 nm after the reaction with Folin-Ciocalteu’s (Sigma-Aldrich, USA) phenol reagent, according to the technique described by Salehi et al. [21] . The measurement was compared with a standard calibration curve of gallic acid solution and the result was reported as μg GAE/ml of QTI samples.
5
Vascular Function and Oxidative Stress Assays
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Phenylephrine hydrochloride (Sigma, cod. P6126), Acetylcholine chloride (Sigma, cod. A6625), Nω-Nitro-L-arginine methyl ester hydrochloride (Sigma, cod. N5751), charybdotoxin (Sigma, cod. C7802), indomethacin (Sigma, cod. I7378), apamin (Sigma, cod. A1289), Dihydroethidium (Sigma, cod. D7008), 2,2-Diphenyl-1-picrylhydrazyl (Sigma, cod. D9132), Folin-Ciocalteu’s (Sigma, cod. 47,641), ascorbic acid, gallic acid, from Sigma-Aldrich®, 4′,6-Diamidino-2-Phenylindole, Dihydrochloride (DAPI) from Invitrogen Molecular Probes™ (cod. D1306) and Dako Fluorescence Mounting Medium (cod. S3023) Na2CO3, NaCl, KCl, KH2PO4, NaHCO3, C6H12O6, CaCl2, MgSO4, ethylenediaminetetraacetic Acid (EDTA), ethanol, methanol, from Vetec®.
6
Quantification of Polyphenols in Extracts
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The extract was prepared with 250 mg of lyophilized sample diluted in 10 mL of MeOH/water (70:30 v/v) centrifuged at 4400 rpm for 20 min and filtered15 (link).
Aliquot of 1 mL of the sample was placed in a test tube and diluted with 10 mL of distilled water. Then, 1.5 mL Folin Ciocalteu's (Sigma) reagent was added and incubated at room temperature for 5 min. 4 mL of 20% (w/w) Na2CO3 were added, adjusted with distilled water up to 35 mL, agitated, and left for 30 min at room temperature. The sample absorbance was measured at 765 nm against a blank, i.e., distilled water16 (link).
Aliquot of 1 mL of the sample was placed in a test tube and diluted with 10 mL of distilled water. Then, 1.5 mL Folin Ciocalteu's (Sigma) reagent was added and incubated at room temperature for 5 min. 4 mL of 20% (w/w) Na2CO3 were added, adjusted with distilled water up to 35 mL, agitated, and left for 30 min at room temperature. The sample absorbance was measured at 765 nm against a blank, i.e., distilled water16 (link).
7
Antioxidant Capacity Evaluation Protocol
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Acetic acid, acetonitrile, Dimethyl sulfoxide, Folin-Ciocalteu's, MTT dye, methanol, Trolox (6 hydroxy-2,5,7,8-tetramethylchroman 2-carboxylic acid) and TPTZ (2,4,6-tripyridyl-s-triazine) were purchased from Sigma Aldrich (St. Louis, MO, USA). All cell culture materials were purchased from Multicell Wisent (Quebec, Canada).
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