5′-GTP was purchased from Promega. [α-32P]-GTP was obtained from PerkinElmer and cyclic di-GMP and pGpG were from Biolog. Restriction endonucleases and Phusion High Fidelity polymerase were purchased from New England Biolabs. PfuTurbo was obtained from Agilent. Qiagen was the source for nickel–nitriloacetic acid (Ni–NTA) agarose. A. vitis S4 cells were a generous gift from Professor Thomas Burr (Cornell University).
Cyclic di gmp
Manufactured by Biolog
Cyclic di-GMP is a bacterial second messenger molecule that plays a crucial role in the regulation of various cellular processes, including biofilm formation, motility, and virulence. It serves as a central signaling molecule in many bacterial species, coordinating their physiological responses to environmental cues.
Automatically generated - may contain errors
Lab products found in correlation
Anti β actin, by Merck Group (2 mentions)
Anti flag, by Merck Group (2 mentions)
Anti phospho irf3, by Cell Signaling Technology (2 mentions)
Anti cgas, by Cell Signaling Technology (2 mentions)
Anti irf3, by Cell Signaling Technology (2 mentions)
Hsv 1 kos strain, by American Type Culture Collection (2 mentions)
Anti calreticulin, by Abcam (2 mentions)
Anti tbk1, by Abcam (2 mentions)
Cgamp, by Biolog (2 mentions)
Anti phospho tbk1, by Cell Signaling Technology (2 mentions)
α 32p gtp, by PerkinElmer (1 mentions)
Restriction endonuclease, by New England Biolabs (1 mentions)
Phusion high fidelity polymerase, by New England Biolabs (1 mentions)
Pfuturbo, by Agilent Technologies (1 mentions)
3 protocols using cyclic di gmp
1
Biochemical Characterization of c-di-GMP
2
Sting and cGAS Knockout MEF Cell Isolation
3
Sting and cGAS Knockout MEF Cell Isolation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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All reagents were purchased from SIGMA unless specified. Primary Sting−/− MEF cells were prepared as previously described26 (link). Primary cGas−/− MEF cells were isolated from cGas−/− mice that were kindly gifted from Dr. Herbert “Skip” Virgin, Washington University School of Medicine in St. Louis. Poly I:C was purchased from American Biosciences. dsDNA (ISD90-mer) was prepared as previously described29 (link). To make biotin-labeled dsDNA (ISD90-mer), 5′-biotin labeled ISD90-mer sense DNA and unlabeled ISD90-mer antisense DNA that were synthesized at SIGMA were annealed in a water bath at 70 °C for 30 min and then cooled in the water bath to room temperature. cGAMP and cyclic di-GMP were purchased from BioLog. HSV-1 (KOS strain) was purchased from ATCC. HSV-1 γ34.5 was kindly provided by Bernard Roizman, University of Chicago. The viral titer was determined by plaque assay using Vero cells as previously described29 (link). Anti-STING rabbit polyclonal antibody was prepared as previously described26 (link). Other antibodies used in this paper are as follows: anti-β-actin (SIGMA, A5441); anti-FLAG (SIGMA, F1804); anti-calreticulin (Abcam, ab14234); anti-IRF3 (Cell Signaling, 4302); anti-phospho-IRF3 (Cell Signaling, 4947); anti-TBK1 (Abcam, ab40676); anti-phospho-TBK1 (Cell Signaling, 5483); anti-cGAS (Cell Signaling, 15102).
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