Iodine-stained visualization of starch was conducted as described [38 (link)]. Briefly, whole Arabidopsis rosettes of four- or six-week-old plants were collected and decolorized using 90% (v/v) of hot ethanol. Ethanol was removed by rinsing in water before staining Lugol’s iodine Reagent (Sigma-Aldrich, Saint-Louis, MO, USA). Over 6-h iodine staining, plants were destained in water until optimal visualization of the amylose–iodine complex in starch was achieved.
Lugol s iodine reagent
Manufactured by Merck Group
Sourced in United States, Germany
Lugol's iodine reagent is a laboratory solution composed of iodine and potassium iodide in water. It is a commonly used reagent in various analytical and diagnostic procedures.
Automatically generated - may contain errors
2 protocols using lugol s iodine reagent
1
Visualizing Starch in Arabidopsis Rosettes
2
Bacterial Inoculum Preparation and Preservation
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Nutrient broth medium (OXOID CM0003B) was purchased from Oxoid, Basingstoke, England, and was used for bacteria inoculum preparation and its agar form to preserve the strains. Lugol's iodine reagent, phosphate buffer solution, HCl, calcium chloride, serum albumin (BSA), soluble starch (Sigma S-2630), and sodium alginate was purchased from Sigma, Aldrich, Germany. All chemicals were analytical grade.
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