Anti nurr77

Manufactured by Novus Biologicals

Anti-Nurr77 is a laboratory reagent used in research applications. It is an antibody that specifically binds to the Nurr1 (NR4A2) transcription factor. Nurr1 is involved in the regulation of gene expression and plays a role in various cellular processes. The Anti-Nurr77 reagent can be utilized in experiments to study the localization, expression, and function of Nurr1 in different cell types and biological systems.

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Lab products found in correlation

Anti phospho creb s133, by Cell Signaling Technology (2 mentions) Anti o glcnac, by Abcam (2 mentions) Anti actin, by Merck Group (2 mentions) Anti rsk1 rsk2 rsk3, by Cell Signaling Technology (2 mentions) Anti phospho erk1 t202 y204 erk2 t185 y187, by R&D Systems (2 mentions) Anti erk1 erk2, by R&D Systems (2 mentions) Anti phospho p90rsk s380, by Cell Signaling Technology (2 mentions) Anti phospho nurr77 s351, by Cell Signaling Technology (2 mentions) Anti creb, by Cell Signaling Technology (2 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions) Dual luciferase assay protocol, by Promega (1 mentions)

2 protocols using anti nurr77

Western Blot Antibody Protocol

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Antibodies for Western blotting were used as follows: anti-O-GlcNAc (No. ab2739-Abcam): 1:1000; Anti-actin (No. A2066, Sigma Aldrich), 1:1000; Anti-Phospho-ERK1(T202/Y204)/ERK2(T185/Y187) (No. AF1018, R&D Systems), 1:500; Anti-ERK1/ERK2 (No. AF1576, R&D Systems), 1:500; Anti Phospho-p90RSK (S380) (No. 9341S, Cell Signaling), 1:500; Anti-Rsk1/rsk2/rsk3 (No. 9355T, Cell Signaling), 1:500; Anti-Phospho-Nurr77 (S351) (No. 5095S, Cell Signaling), 1:500; Anti-Nurr77 (No. NB100-56745, Novus Biologicals), 1:500; Anti-Phospho-CREB (S133) (No. 9198S, Cell Signaling), 1:500; Anti-CREB (No. 9104S, Cell Signaling), 1:500.

Massman L.J., Pereckas M., Zwagerman N.T, & Olivier-Van Stichelen S. (2021). O-GlcNAcylation Is Essential for Rapid Pomc Expression and Cell Proliferation in Corticotropic Tumor Cells. Endocrinology, 162(12), bqab178.

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Luciferase Assay for Pomc Promoter

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Antibodies for western blotting are used as follows: anti-O-GlcNAc (#ab2739-Abcam): 1:1000; Anti-actin (#A2066, Sigma Aldrich), 1:1000;
Anti-Phospho-ERK1(T202/Y204)/ERK2(T185/Y187) (#AF1018, R&D systems), 1:500; Anti-ERK1/ERK2 (#AF1576,R&D systems), 1:500; Anti Phospho-p90RSK (S380) (#9341S, Cell Signaling), 1:500; Anti-Rsk1/rsk2/rsk3 (#9355T, Cell Signaling), 1:500;
Anti-Phospho-Nurr77 (S351) (#5095S, Cell Signaling), 1:500; Anti-Nurr77 (#NB100-56745, Novus Biologicals), 1:500; Anti-Phospho-CREB (S133) (#9198S, Cell Signaling), 1:500; Anti-CREB (#9104S, Cell Signaling), 1:500.
AtT-20 cells were concurrently transfected using Lipofectamine 3000 (Thermofisher) with two plasmids -one containing the pRL Renilla luciferase gene under the control of the CMV promoter (# E2261, Promega) and the other containing the firefly luciferase gene under the control of the Pomc promoter (-646bp to +65bp) (#17553, Addgene). Forty-eight hours after transfection, luciferase activity was measured using the dual-luciferase assay protocol (#E1910, Promega). Transfection was performed 48h before measurement of the promoter activity.

Massman L., Pereckas M., Zwagerman N.T., & Stichelen S.O. (2021). O-GlcNAcylation is essential for rapid Pomc expression and cell proliferation in corticotropic tumor cells.

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