Acetonitrile with hplc grade

Ferulic acid (Batch No. 110773-200611) was obtained from the National Institutes for Food and Drug Control (Beijing, China). Chlorogenic acid (Batch No. MUST-17030620) was obtained from Chengdu Manchester biotech Co., Ltd. (Chengdu, China). Lobetyolin (Batch No. 16022204), Andrographolide (Batch No. 16073103), and dehydrated andrographolide (Batch No. 16022501) were obtained from Chengdu Zhuo Pu Instrument Co., Ltd (Chengdu, China). Fukeqianjin tablets were obtained from Zhuzhou Qianjin pharmaceutical Co., Ltd. (Hunan, China). Azithromycin (Batch No. R37977) was purchased from Pfizer pharmaceutical Co., Ltd. (NY, USA). Staphylococcus aureus (ATCC25923) and E. coli were purchased from Chinese national fungus storehouse. Methanol and acetonitrile with HPLC-grade were purchased from Thermo Fisher Scientific Inc. (Iowa, USA). Formic acid was purchased from Sigma Chemical Co. (St. Louis, MO, USA). Water used in this study was prepared by ULUP Ultrapure Water System (Chengdu, China).

Puerarin with the batch number of 110752-200912 was bought from NICPBP (China). Daidzin (MUST-15031507), daidzein (MUST-1505-1511), genistin (MUST-14110908), and genistein (MUST-15021802) were bought from the Chengdu Must Bio-Technology Co., Ltd. (Chengdu, China). STZ with the batch number of SLBB7526V were obtained from Sigma Chemical Co. (St. Louis, MO, USA). Water used in this study was prepared by ULUP Ultrapure Water System (Ulupure, Chengdu, China). Methanol and acetonitrile with HPLC-grade were purchased from Thermo Fisher Scientific Inc. (Iowa, USA).

Fluoren-9-ylmethoxycarbonyl (Fmoc) amino acid derivatives with side-chain protections were purchased from the GL Biochem Co., Ltd. (Shanghai, China). 2-Nitrophenyl β-d-galactopyranoside (ONPG), oxidized nicotinamide adenine dinucleotide (NAD⁺), 6-phosphogluconate, iodonitrotetrazolium chloride (INT) and reagents for peptide synthesis were purchased from the Aladdin Industrial Corporation (Shanghai, China). Regents about ethylenediaminetetraacetic acid (EDTA), sodium deoxycholate, hexadecyltrimethylammonium bromide (CTAB), beta-mercaptoethanol, sodium salts, and dimethylformamide (DMF) were obtained from the Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). Muller-Hinton Broth (MHB) was purchased from the Beijing Ao Bo Xing Bio-Tech (Beijing, China). Agar powder and Tris were purchased from Gene Company Co., Ltd. (Hong Kong, China). ZoRBAX SB-C18 PreHT Column was purchased from the Agilent Technologies Inc. (Santa Clara, CA, USA). Acetonitrile with HPLC grade was purchased from the Thermo Fisher Scientific Inc. (Shanghai, China). Bovine Serum Albumin (BSA) was purchased from Beijing Solarbio Science and Technology Co., Ltd. (Beijing, China).