Recombinant tissue type plasminogen activator

Manufactured by Innovative Research

Sourced in United States

Recombinant tissue-type plasminogen activator (tPA) is a laboratory-produced protein that plays a key role in the breakdown of blood clots. It functions by converting the inactive proenzyme plasminogen into the active enzyme plasmin, which then degrades fibrin, the main component of blood clots.

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Lab products found in correlation

Synergy h1 spectrophotometer, by Agilent Technologies (2 mentions) Ovalbumin (ova), by Merck Group (2 mentions) Human alpha thrombin, by Prolytix (2 mentions) Direct q 3 system, by Merck Group (2 mentions) Acetic acid, by BD (1 mentions) Heparinase, by New England Biolabs (1 mentions) Mineral oil, by Thermo Fisher Scientific (1 mentions) Acetic acid, by Thermo Fisher Scientific (1 mentions)

2 protocols using recombinant tissue type plasminogen activator

Fibrinogen Clotting Kinetics Assay

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Human plasminogen-free fibrinogen was purchased from Aniara Diagnostica (West Chester, OH). Ovalbumin (98% purity, grade V) was purchased from Millipore-Sigma (St. Louis, MO). Re-lipidated tissue factor (TF) (Dade Innovin, B4212-40 Siemens Healthcare, Erlangen, Germany), acetic acid (glacial, 100%, reagent grade), mineral oil (light, C₁₆H₁₀N₂Na₂O₇S₂, reagent grade), and 96-well microplates (polystyrene, flat bottom, nontissue culture–treated plate, Falcon) were from Fisher Scientific (Hampton, NH). Human alpha thrombin was purchased from Haematologic Technologies (Essex Junction, VT). Recombinant tissue-type plasminogen activator (tPA) was from Innovative Research (Novi, MI). Heparinase was from New England Biolabs (Ipswich, MA). Distilled water was prepared using a Direct Q3 system (Millipore-Sigma, St Louis, MO). Tris-buffered saline (10×, 500-mM Tris, 1500-mM NaCl) was prepared in house, and the pH was adjusted to 7.4. Synthetic phospholipid vesicles (phosphatidylserine: phosphatidylethanolamine: phosphatidylcholine ratios of 15:41:44) were a gift from Dougald Monroe, University of North Carolina. For kinetic optical density measurements, a Synergy H1 spectrophotometer (BioTek, Winooski, VT) was used.

Ilich A., Gernsheimer T.B., Triulzi D.J., Herren H., Brown S.P., Holle L.A., Lucas A.T., de Laat B., El Kassar N., Wolberg A.S., May S, & Key N.S. (2022). Absence of hyperfibrinolysis may explain lack of efficacy of tranexamic acid in hypoproliferative thrombocytopenia. Blood Advances, 7(6), 900-908.

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Fibrinogen-based Coagulation Assay

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Human plasminogen-free fibrinogen was purchased from Aniara Diagnostica (West Chester, OH, USA). Ovalbumin (98% purity, grade V) was purchased from Millipore-Sigma (St. Louis, MO, USA). Re-lipidated tissue factor (TF) (Dade Innovin, B4212–40 Siemens Healthcare, Erlangen, Germany), acetic acid (glacial, 100%, reagent grade), mineral oil (light, C₁₆H₁₀N₂Na₂O₇S₂, reagent grade), and 96-well microplates (polystyrene, flat bottom, non-tissue culture treated plate, Falcon) were purchased from Fisher Scientific (Hampton, NH, USA). Human alpha thrombin was purchased from Haematologic Technologies (Essex Junction, VT, USA). Recombinant tissue-type plasminogen activator (tPA) was from Innovative Research (Novi, MI, USA). Distillated water (diH₂O) was prepared using a Direct Q3 system (Millipore-Sigma, St. Louis, MO, USA). Tris Buffered Saline (10x, 500 mM Tris, 1500 nM NaCl) was prepared in house and the pH was adjusted to 7.4. Synthetic phospholipid vesicles (PS:PE:PC ratios of 15:41:44) were a gift from Dr. Dougald Monroe, University of North Carolina. For kinetic optical density measurements, a Synergy H1 spectrophotometer (BioTek, Winooski, VT, USA) was used.

Ilich A., Kumar V., Ferrara M.J., Henderson M.W., Noubouossie D.F., Jenkins D.H., Kozar R.A., Park M.S, & Key N.S. (2021). Euglobulin Clot Lysis Time Reveals a High Frequency of Fibrinolytic Activation in Trauma. Thrombosis research, 204, 22-28.

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