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Cd21 buv737

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The CD21 BUV737 is a fluorochrome-conjugated antibody that recognizes the CD21 surface antigen, which is expressed on mature B cells and a subset of T cells. The CD21 BUV737 can be used in flow cytometry applications to identify and enumerate these cell populations.

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Lab products found in correlation

Aqua viability dye, by Thermo Fisher Scientific (2 mentions) Recombinant bir a, by Avidity (2 mentions) Lightning link apc conjugation kit, by Abcam (2 mentions) Cd14 bv510, by BioLegend (1 mentions) Cd16 bv 510, by BioLegend (1 mentions) Lsrii fortessa, by BD (1 mentions) Cd3 bv510, by BioLegend (1 mentions) Cd27 bv605, by BioLegend (1 mentions) Cd10 bv510, by BioLegend (1 mentions) Cd8a bv510, by BioLegend (1 mentions) Cd20 apc cy7, by BD (1 mentions) Streptavidin phycoerythrin pe, by Thermo Fisher Scientific (1 mentions) Igg bv786, by BD (1 mentions) Streptavidin bv510, by BD (1 mentions) Cd19 ecd, by Beckman Coulter (1 mentions) Igd pe cy7, by BD (1 mentions) Cd20alexa700, by BD (1 mentions) Cd10 bv510 hi10a, by BioLegend (1 mentions) Cd27 bv605 o323, by BioLegend (1 mentions) Cd19 ecd j3 119, by Beckman Coulter (1 mentions)

2 protocols using cd21 buv737

1

SARS-CoV-2 Spike and RBD Staining of PBMCs

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PBMCs were stained with SARS-CoV-2 Spike and RBD probes, as previously described (Juno et al., 2020 (link)). Probes were generated by sequential addition of streptavidin-phycoerythrin (PE) (Thermo Fisher Scientific) to trimeric S protein biotinylated using recombinant Bir-A (Avidity), while SARS-CoV-2 RBD was labeled to APC using an APC Conjugation Lightning-Link Kit (Abcam). PBMCs were surface stained with Aqua viability dye (Thermo Fisher) and monoclonal antibodies against CD19-ECD (#IM2708U, Beckman Coulter), IgM BUV395 (#563903), CD21 BUV737 (#564437), IgD PE-Cy7 (#561314), IgG BV786 (#564230), streptavidin-BV510 (#563261) (BD Biosciences), CD20 APC-Cy7 (#302314), CD14 BV510 (#301841), CD3 BV510 (#317332), CD8a BV510 (#301048), CD16 BV510 (#302048), CD10 BV510 (#312220) and CD27 BV605 (#302829) (BioLegend). Cells were washed, fixed with 1% formaldehyde and acquired on a BD LSRII Fortessa.
Nguyen T.H., Rowntree L.C., Petersen J., Chua B.Y., Hensen L., Kedzierski L., van de Sandt C.E., Chaurasia P., Tan H.X., Habel J.R., Zhang W., Allen L.F., Earnest L., Mak K.Y., Juno J.A., Wragg K., Mordant F.L., Amanat F., Krammer F., Mifsud N.A., Doolan D.L., Flanagan K.L., Sonda S., Kaur J., Wakim L.M., Westall G.P., James F., Mouhtouris E., Gordon C.L., Holmes N.E., Smibert O.C., Trubiano J.A., Cheng A.C., Harcourt P., Clifton P., Crawford J.C., Thomas P.G., Wheatley A.K., Kent S.J., Rossjohn J., Torresi J, & Kedzierska K. (2021). CD8+ T cells specific for an immunodominant SARS-CoV-2 nucleocapsid epitope display high naive precursor frequency and TCR promiscuity. Immunity, 54(5), 1066-1082.e5.
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2

Workflow for SARS-CoV-2 S-specific B Cell Profiling

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Probes for delineating SARS-CoV-2 S-specific B cells within cryopreserved human PBMC were generated by sequential addition of streptavidin-PE (Thermofisher) to trimeric S protein biotinylated using recombinant Bir-A (Avidity). SARS-CoV-2 RBD protein was directly labelled to APC using an APC Conjugation Lightning-link kit (Abcam). Cells were stained with Aqua viability dye (Thermofisher). Monoclonal antibodies for surface staining included: CD19-ECD (J3-119) (Beckman Coulter), CD20 Alexa700 (2H7), IgM-BUV395 (G20-127), CD21-BUV737 (B-ly4), IgD-Cy7PE (IA6-2), IgG-BV786 (G18-145) (BD), CD14-BV510 (M5E2), CD3-BV510 (OKT3), CD8a-BV510 (RPA-T8), CD16-BV510 (3G8), CD10-BV510 (HI10a), CD27-BV605 (O323) (Biolegend), IgA-Vio450 (clone) (Miltenyi). Cells were washed, fixed with 1% formaldehyde (Polysciences) and acquired on a BD LSR Fortessa or BD Aria II. Gating is shown in Supplementary Fig. 4.
Wheatley A.K., Juno J.A., Wang J.J., Selva K.J., Reynaldi A., Tan H.X., Lee W.S., Wragg K.M., Kelly H.G., Esterbauer R., Davis S.K., Kent H.E., Mordant F.L., Schlub T.E., Gordon D.L., Khoury D.S., Subbarao K., Cromer D., Gordon T.P., Chung A.W., Davenport M.P, & Kent S.J. (2021). Evolution of immune responses to SARS-CoV-2 in mild-moderate COVID-19. Nature Communications, 12, 1162.
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