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Immage automatic immunoassay system

Manufactured by Beckman Coulter
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The IMMAGE automatic immunoassay system is a laboratory instrument designed for the automated detection and measurement of various analytes in biological samples. It utilizes immunoassay technology to perform quantitative analysis of proteins, hormones, and other molecules of clinical importance.

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Lab products found in correlation

Architect insulin, by Abbott (3 mentions) Procartaplex mix match human 13 plex, by Thermo Fisher Scientific (2 mentions) Human il 6 quantikine hs elisa kit, by R&D Systems (2 mentions) Unicel dxc 800 synchron, by Beckman Coulter (1 mentions) Human leptin quantikine elisa kit, by R&D Systems (1 mentions) Discovery w, by Hologic (1 mentions)

4 protocols using immage automatic immunoassay system

1

Biomarker Measurement in Fasting Plasma

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Fasting blood samples were drawn from each participant and transferred on ice to center laboratory for analysis. An IMMAGE automatic immunoassay system (Beckmann-Coulter) was used to examine plasma level of Hs-CRP. Intra-assay variability coefficient lied between 3.5-5.0%, and inter-assay variability coefficient between 4.0-7.5%, and limited detection level was 0.2 mg/L. APN was measured by a high sensitivity Human adiponectin ELISA kit (BioCat GmbH) with intra-assay variability coefficient lied between 4.0-5.5% and inter-assay variability coefficient between 5.0-7.5%. Triglyceride (TG), total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C), high density lipoprotein-cholesterol (HDL-C) and fasting blood glucose were also measured by Automatic Biochemistry Analyzer (Beckman coulter UniCel DxC 800 Synchron).
Liao H., Li Z., Zheng D., Liu J., Liu Y., Xiao C, & Wang H. (2014). Increased Hs-CRP/adiponectin ratio is associated with increase carotid intima-media thickness. Lipids in Health and Disease, 13, 120.
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2

Metabolic and Inflammatory Markers in Cardiometabolic Diseases

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Laboratory parameters were assessed using standard analytical methods. Hypertension was diagnosed by a systolic/diastolic blood pressure >140/90 mmHg and or a prescribed anti-hypertensive treatment and T2D was diagnosed by a fasting blood glucose > 7.0 mmol/l, a random glucose measurement > 11.0 mmol/l or a HbA1c > 6.5% or a prescribed glucose-lowering drug, both defined according to the American Diabetes Association criteria.
Blood samples were collected after an overnight fast. Fasting serum glucose, triglycerides, and HbA1c were measured using enzymatic methods. Fasting serum insulin and C-peptide were measured using a chemiluminescence assay (Insulin Architect, Abbott). High-sensitivity C-reactive protein (CRP) was measured using an IMMAGE automatic immunoassay system (Beckman-Coulter) and high-sensitivity interleukin 6 (hs-IL-6) was measured using the Human IL-6 Quantikine HS ELISA Kit (R&D Systems). IFN-γ–induced protein 10 (IP-10), interleukin 7 (IL-7), macrophage migration inhibitory factor (MIF), C-X-C motif chemokine ligand 2 (CXCL2), and 5 (CXCL5) were measured by using a Luminex assay (ProcartaPlex Mix&-Match Human 13-plex; eBioscience, San Diego, CA, USA).
Bouazizi K., Zarai M., Noufaily A., Prigent M., Dietenbeck T., Bollache E., Nguyen T., Della Valle V., Blondiaux E., Clément K., Aron-Wisnewsky J., Andreelli F., Redheuil A, & Kachenoura N. (2023). Associations of aortic stiffness and intra-aortic flow parameters with epicardial adipose tissue in patients with type-2 diabetes. Frontiers in Clinical Diabetes and Healthcare, 4, 1106342.
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3

Comprehensive Obesity Assessment Protocol

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Subjects underwent systematic explorations that included a thorough medical interview recording obesity comorbidities such as type 2 diabetes, a routine physical examination and fasting biological measurements as previously described.18 21 Blood samples were collected after an overnight fast. Fasting glucose, high density lipoprotein cholesterol (HDL-c), triglycerides and haemoglobin A1c (HbA1c) were measured using enzymatic methods. Fasting serum insulin was measured using a chemiluminescence assay (Insulin Architect, Abbott). Serum leptin was measured using the Human Leptin Quantikine ELISA Kit (R&D Systems, Inc). High-sensitivity c-reactive protein was measured using an IMMAGE automatic immunoassay system (Beckman-Coulter).
Weight and height were assessed during the clinical inclusion visit according to standardised procedures using the same scale for all subjects. Body composition was assessed using the same device; a whole-body fan-beam DXA scan (Hologic Discovery W, software V.12.6, 2; Hologic, Bedford, Massachusetts) which evaluated per cent body fat mass. BFMI was calculated as body fat (kg)/height (m2).
Olsson L.M., Poitou C., Tremaroli V., Coupaye M., Aron-Wisnewsky J., Bäckhed F., Clément K, & Caesar R. (2019). Gut microbiota of obese subjects with Prader-Willi syndrome is linked to metabolic health. Gut, 69(7), 1229-1238.
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4

Biomarker Evaluation in Fasting Samples

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Blood samples were collected after an overnight fast. Fasting serum glucose, triglycerides, and HbA1c were measured using enzymatic methods. Fasting serum insulin and C-peptide were measured using a chemiluminescence assay (Insulin Architect, Abbott). High-sensitivity C-reactive protein (hs-CRP) was measured using an IMMAGE automatic immunoassay system (Beckman-Coulter) and high-sensitivity interleukin 6 (hs-IL-6) was measured using the Human IL-6 Quantikine HS ELISA Kit (R&D Systems). IFN-γ–induced protein 10 (IP-10), interleukin 7 (IL-7), C-X-C motif chemokine ligand 2 (CXCL2), and 5 (CXCL5) were measured by using a Luminex assay (ProcartaPlex Mix&Match Human 13-plex; eBioscience, San Diego, CA, USA).
Molinaro A., Bel Lassen P., Henricsson M., Wu H., Adriouch S., Belda E., Chakaroun R., Nielsen T., Bergh P.O., Rouault C., André S., Marquet F., Andreelli F., Salem J.E., Assmann K., Bastard J.P., Forslund S., Le Chatelier E., Falony G., Pons N., Prifti E., Quinquis B., Roume H., Vieira-Silva S., Hansen T.H., Pedersen H.K., Lewinter C., Sønderskov N.B., Køber L., Vestergaard H., Hansen T., Zucker J.D., Galan P., Dumas M.E., Raes J., Oppert J.M., Letunic I., Nielsen J., Bork P., Ehrlich S.D., Stumvoll M., Pedersen O., Aron-Wisneswky J., Clément K, & Bäckhed F. (2020). Imidazole propionate is increased in diabetes and associated with dietary patterns and altered microbial ecology. Nature Communications, 11, 5881.
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