ChIP analysis was essentially performed as described previously (Frank et al., 2001 (link)). ChIP reactions were performed using the following antibodies: anti-GFP (ab290, Abcam), anti-CBX8 (C15410333, Diagenode), anti-EZH2 (D2C9, Cell Signaling), anti-H2AK119ub (D27C4, Cell Signaling), anti-H3K27me3 (C15410195, Diagenode), anti-H3K27Ac (C15410196, Diagenode), anti-H3K4me1 (C15410194, Diagenode), and anti-H3K4me3 (C15410003, Diagenode). ChIP efficiencies were assessed using qPCR. Primer sequences can be found in Supplementary file 6 .
Anti cbx8
Manufactured by Diagenode
The Anti-CBX8 is a laboratory reagent used for the detection and quantification of the CBX8 protein in biological samples. It is a specific antibody designed to bind to and identify the CBX8 protein, which is involved in various cellular processes. The Anti-CBX8 can be used in techniques such as Western blotting, immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA) to study the expression and localization of the CBX8 protein in research settings.
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Lab products found in correlation
Anti ezh2 d2c9, by Cell Signaling Technology (2 mentions)
Anti h3k4me3, by Diagenode (1 mentions)
Anti h3k27ac, by Diagenode (1 mentions)
Anti h2ak119ub d27c4, by Cell Signaling Technology (1 mentions)
Anti h3k4me1, by Diagenode (1 mentions)
Anti h3k27me3, by Diagenode (1 mentions)
Ab5821, by Abcam (1 mentions)
Ab181140, by Abcam (1 mentions)
Ab8580, by Abcam (1 mentions)
Ab290, by Abcam (1 mentions)
Anti β actin c4, by Santa Cruz Biotechnology (1 mentions)
Anti h3k27me3, by Merck Group (1 mentions)
Spa 810, by Enzo Life Sciences (1 mentions)
Anti h2ak119ub, by Cell Signaling Technology (1 mentions)
2 protocols using anti cbx8
1
ChIP Analysis Protocol for Epigenetic Markers
2
Comprehensive Protein Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Western blot analysis was performed as published previously (van den Boom et al., 2013 (link)). The following antibodies were used: anti-GFP (ab290, Abcam), anti-EZH2 (D2C9, Cell Signaling Technology), anti-SUZ12, anti-CBX4 (09–029, Merck), anti-CBX8 (C15410333, Diagenode), anti-RING1B (ab181140, Abcam), anti-BMI1 (F6, Merck), anti-DNAJB1 (SPA-400, Enzo Life Sciences), anti-HSP70 (SPA-810, Enzo Life Sciences), anti-Fibrillarin (ab5821, Abcam), anti-H3K27me3 (07–449, Merck), anti-H2AK119ub (D27C4, Cell Signaling Technology), anti-H3K4me3 (ab8580, Abcam), and anti-β-Actin (C4, Santa Cruz).
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