Animals (n=3 for treated FSLs and free-GNP injected FSLs) were scanned in-vivo with a micro-CT scanner (Skyscan High Resolution Model 1176, Bruker micro-CT, Kontich, Belgium; nominal resolution of 35μm, a 0.5 mm aluminum filter, and applied x-ray tube voltage of 45 kV; detection limit ~1000 GNP-labeled cells), to screen for the location of the cells one day and one month after injection. Specifications and further details in SI. At the end of the experiment, brains where taken for further ex-vivo scans and FAAS analysis.
Skyscan high resolution model 1176
Manufactured by Bruker
Sourced in Belgium
The Skyscan High Resolution Model 1176 is a compact, high-resolution micro-CT scanner designed for non-destructive 3D imaging of small samples. It offers high-quality data acquisition and reconstructions with a focal spot size of less than 5 microns, enabling high-resolution scans of a variety of samples.
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4 protocols using skyscan high resolution model 1176
1
In-vivo Tracking of GNP-labeled Cells
2
In vivo CT Imaging of GNP-labeled Cells
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In vivo computed tomography (CT) scans were conducted as previously [41 (link)] at 24 h, 72 h, and 1 week after IN administration of PLX-PAD. Animals were scanned in vivo with a micro-CT scanner (Skyscan High Resolution Model 1176, Bruker micro-CT, Kontich, Belgium) at a nominal resolution of 35 μm, using a 0.5 mm aluminum filter and an applied X-ray tube voltage of 60 kV, with current source 350 μA (detection limit ~1000 GNP-labeled cells).
3
In Vivo Imaging of Gold Nanoparticles in Brains
4
In Vivo Imaging of Gold Nanoparticles in Brains
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In vivo scans for detection of the GNPs in brains were performed using a micro-CT scanner (Bruker, Sky scan high-resolution model 1176, Kontich, Belgium) with a nominal resolution of 35 μm, a 0.2 mm aluminum filter, and a tube voltage of 40 kV. Reconstruction was carried out with a modified Feldkamp algorithm using the SkyScan NRecon software (Bruker Skyscan NRecon VI.7.4.2, Kontich, Belgium) accelerated by GPU. Ring artifact reduction, Gaussian smoothing (3%), and beam hardening correction (20%) were applied. Volume rendered 3D images were generated using an RGBA transfer function in SkyScan CT-Voxel (Bruker CTvox V3.3.l, 3D.SUITE software, Kontich, Belgium) software.
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