For GP deglycosylation, supernatants containing EBOV GPs were treated using EDEGLY kit (Sigma) for 48 h according to the manufacturer's instructions. Shed GP mixed with deglycosylation enzymes pretreated at 80°C for 30 min were used as a control. Monocyte-derived DCs and macrophages were treated with deglycosylated shed GP samples for 24 hours and culture supernatants were assayed for the presence of TNFα using a Multiplex cytometric bead array (Bio-Rad) in Luminex MAGPIX.
Multiplex cytometric bead array
Manufactured by Bio-Rad
The Multiplex cytometric bead array is a flow cytometry-based technology that enables the simultaneous quantification of multiple analytes in a single sample. It utilizes a set of beads, each coated with a specific capture antibody, to bind and detect target analytes. The beads are then analyzed using a flow cytometer, allowing for the rapid and sensitive measurement of multiple proteins or other biomolecules in a sample.
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Lab products found in correlation
2 protocols using multiplex cytometric bead array
1
Deglycosylation of Ebola GP Effects
2
Evaluating Innate Immune Responses to EBOV
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Culture supernatants from monocyte-derived DCs or macrophages treated with soluble EBOV GPs for 24 hours were assayed for presence of TNF-α, IL-6, IL-8, IL12p40, IL1β, IL10 and IL1-RA using Multiplex cytometric bead array (Bio-Rad) in Luminex MAGPIX. The assay was performed according to the manufacturer's instructions.
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