Cd19 bv786

Manufactured by BioLegend

CD19-BV786 is a fluorochrome-conjugated antibody that binds to the CD19 antigen. CD19 is a cell surface glycoprotein expressed on B lymphocytes and their malignant counterparts.

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2 protocols using cd19 bv786

Neonatal Lung Immune Cell Profiling

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Neonatal lung single‐cell suspensions were used for all immunostaining. Panels of monoclonal antibodies (purchased from BD Bioscience unless otherwise stated) were developed to enable phenotypic characterisation of leucocytes of myeloid: CD45‐PerCP (clone 30‐F11), CD11b‐v500 (clone M1/70), CD11c‐AF700 (clone HL3), CD19‐BV786 (clone 1D3), CD103‐PE (clone M290), CD301‐PE‐Cy7 (clone MGL1/MGL2; BioLegend), F4/80‐FITC (clone BM8; BioLegend, San Diego), Ly6G/C‐APC‐Cy7 (clone RB6‐8C5), I‐A/I‐E‐AF647 (clone M5/114.14.2) and B220/CD45R‐PE‐CF594 (clone RA3‐6B2) and lymphoid: CD45‐PerCP (clone 30‐F11), NKp46‐PE‐Cy7 (clone 29A1.4; BioLegend), CD19‐BV786 (clone 1D3), CD3‐FITC (clone 17A2), CD4‐v500 (clone RM4‐5), CD8α‐BV650 (clone 53‐6.7), CD25‐APC‐Cy7 (clone PC61) and Foxp3‐PE (clone FJK‐16s) lineages. Intracellular staining for Foxp3 was performed using an intracellular Foxp3/Transcription Factor Staining Buffer Kit (eBioscience, San Diego). All samples were kept as individuals. Immune cell phenotypic characterisation was performed using the FlowJo software (version 10.6.1; BD Bioscience). Fluorescent minus one staining controls were used for all panels where necessary. Flow cytometry data quality was based on primary time gates to ensure appropriate laser delay (pre‐determined by automated CS&T) during sample acquisition.

Lauzon‐Joset J., Mincham K.T., Scott N.M., Khandan Y., Stumbles P.A., Holt P.G, & Strickland D.H. (2021). Protection against neonatal respiratory viral infection via maternal treatment during pregnancy with the benign immune training agent OM‐85. Clinical & Translational Immunology, 10(7), e1303.

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Multicolor Flow Cytometry of THP-1 and Blood

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Flow cytometry analysis of THP-1 cells and total blood was performed using the following antibodies. Conjugated anti-human SR-B1 APC (Miltenyi Biotec 130-111-237), CD235a APC Cy7 (BioLegend 3,49,115), CD45 BUV805 (BD Biosciences 6,12,892), CD56 BUV 737 (BD Biosciences 6,12,767), CD11c PeCy7 (BioLegend 3,37,216), CD11b PE (eBioscience 12-0118-41), CD3 PerCP Cy5.5 (BD Biosciences 5,60,835), CD19 BV786 (BioLegend 3,02,239), HLA-DR BUV 395 (BD Biosciences 7,40,302), CD14 BV605 (BioLegend 3,01,834). Labeling of mouse blood was done using the following conjugated antibodies: CD45.2 PE (eBioscience 12-0454–82), CD115 PerCPeF710 (eBioscience 46-1,152-82), CD11b BV650 (eBioscience 48-0112–20), Ly6G BV510 (BD Biosciences 5,63,402), CD3 APC-eF780 (BioLegend 47-0032–82). Fc receptors were blocked using FCR blocking reagent (Myltenyi Biotec). Surface membrane staining was performed in PBS FBS 2%. The Zombie UV fixable viability dye (BioLegend 4,23,107) was used to exclude dead cells. Samples were acquired on a Cytoflex (Beckman Coulter) and analyzed with FlowJo 10 (BD Biosciences).

Tello Rubio B., Bugault F., Baudon B., Raynal B., Brûlé S., Morel J.D., Saint-Auret S., Blanchard N., Demangel C, & Guenin-Macé L. (2021). Molecular Mechanisms Underpinning the Circulation and Cellular Uptake of Mycobacterium ulcerans Toxin Mycolactone. Frontiers in Pharmacology, 12, 733496.

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