Human no2 inos elisa kit

Manufactured by FineTest

Sourced in China

The Human NO2/iNOS ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay designed for the measurement of nitric oxide (NO) and inducible nitric oxide synthase (iNOS) levels in human samples. The kit utilizes pre-coated microplates and specific antibodies to detect and quantify the target analytes.

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Lab products found in correlation

Infinite m200, by Tecan (1 mentions)

3 protocols using human no2 inos elisa kit

Quantifying Inducible Nitric Oxide Synthase

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To assess inducible nitric oxide synthase (iNOS) expression in plasma, a human NO2/iNOS ELISA kit (catalog number EH0556; FineTest, Wuhan, China) was used. This assay was based on sandwich enzyme-linked immune-sorbent assay technology. NOS2/iNOS protein synthesis was determined using a standard curve.

Levenez M., Lambrechts K., Mrakic-Sposta S., Vezzoli A., Germonpré P., Pique H., Virgili F., Bosco G., Lafère P, & Balestra C. (2022). Full-Face Mask Use during SCUBA Diving Counters Related Oxidative Stress and Endothelial Dysfunction. International Journal of Environmental Research and Public Health, 19(2), 965.

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Inducible Nitric Oxide Synthase Quantification

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To assess inducible nitric oxide synthase (iNOS) expression in plasma, a human NO₂/iNOS ELISA kit (cat no EH0556; FineTest, Wuhan, China) was used. This assay was based on sandwich enzyme-linked immune-sorbent assay technology. NOS₂/iNOS protein synthesis was determined using a standard curve. Samples and standards were read at a wavelength of 450 nm, and the analysis was carried out according to the manufacturer’s instructions.

Bosco G., Paganini M., Giacon T.A., Oppio A., Vezzoli A., Dellanoce C., Moro T., Paoli A., Zanotti F., Zavan B., Balestra C, & Mrakic-Sposta S. (2021). Oxidative Stress and Inflammation, MicroRNA, and Hemoglobin Variations after Administration of Oxygen at Different Pressures and Concentrations: A Randomized Trial. International Journal of Environmental Research and Public Health, 18(18), 9755.

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Quantifying Nitric Oxide Pathway

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Nitrite and Nitrate (NOx) concentrations were determined on urine with a colorimetric method based on the Griess reaction, using a commercial kit (Cayman Chemical, Ann Arbor, MI, USA) as previously described [76 (link),79 (link)].
iNOS expression in plasma was assessed by a human NO₂/iNOS ELISA kit (catalogue no.: EH0556; FineTest, Wuhan, China). This assay was based on the sandwich enzyme-linked immune-sorbent assay technology.
All the samples were read in duplicate, with each at a correct wavelength by a spectrophotometer microplate reader (Infinite M200, Tecan Group Ltd., Männedorf, Switzerland), according to the manufacturer’s instructions.

Balestra C., Lambrechts K., Mrakic-Sposta S., Vezzoli A., Levenez M., Germonpré P., Virgili F., Bosco G, & Lafère P. (2021). Hypoxic and Hyperoxic Breathing as a Complement to Low-Intensity Physical Exercise Programs: A Proof-of-Principle Study. International Journal of Molecular Sciences, 22(17), 9600.

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