Body composition was analyzed by NMR (Minispec LF50; Bruker). Glucose tolerance was determined after 16 h of fasting and the intraperitoneal injection of glucose (2 g/kg) and repeated blood glucose measurements from the tail vein using glucose test strips (Contour Next; Bayer). Serum insulin was measured by ELISA (Rat Insulin ELISA; Crystal Chem). Serum NEFA and triglycerides were analyzed biochemically using commercial NEFA-HR (2 (link)) (FUJIFILM Wako Chemicals Europe) and triglycerides FS (DiaSys Diagnostic Systems) kits, respectively. Serum β-hydroxybutyrate was analyzed by test strips (FreeStyle precision pro; Abbott). Serum FGF21 was determined by ELISA (Quantikine ELISA mouse/rat FGF-21; R&D Systems). Food intake was determined in single-housed mice for 24 h.
Freestyle precision pro
Manufactured by Abbott
Sourced in United Kingdom
The FreeStyle Precision Pro is a portable blood glucose monitoring system designed for professional use. It provides accurate measurements of blood glucose levels, enabling healthcare professionals to effectively monitor and manage their patients' diabetes.
Automatically generated - may contain errors
Lab products found in correlation
Minispec lf50, by Bruker (1 mentions)
Triglycerides fs, by DiaSys (1 mentions)
Contour next, by Bayer (1 mentions)
Quantikine elisa mouse rat fgf 21, by R&D Systems (1 mentions)
Rat insulin elisa, by Crystal Chem (1 mentions)
Atellica, by Siemens (1 mentions)
Enlite glucose sensor, by Medtronic (1 mentions)
Ipro2, by Medtronic (1 mentions)
4 protocols using freestyle precision pro
1
Comprehensive Metabolic Phenotyping in Mice
2
Evaluating Body Composition and Metabolic Changes
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Changes in body composition (total‐, fat‐ and lean body weight) and body fat distribution (the subdivision of abdominal adipose tissue into its visceral and subcutaneous subcompartments) were evaluated using dual X‐ray absorptiometry on the Hologic Discovery scanner (Hologic Inc., Marlborough, MA, USA). Participants were measured at baseline and after 13 weeks of treatment. The biometabolic blood profile consisted of clinical biomarkers of metabolic function [beta‐hydroxybutyrate (βOHB), haemoglobin A1c (HbA1c), fasting glucose, area under the curve derived from a standardized 2‐h oral glucose tolerance test, C‐peptide, triglycerides, total cholesterol, and low‐density‐lipoprotein and high‐density lipoprotein]. βOHB was acquired at baseline and week 13 using capillary blood samples and immediately analysed using the Freestyle Precision Pro (Abbott Laboratories Limited, Maidenhead, Berks, UK). The remaining biomarkers were acquired from venous samples at baseline and week 13, with biomarkers processed immediately after acquisition using automatic enzymatic light absorptiometry (Atellica; Siemens, Germany). HbA1c was reported using the standard from the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC; mmol/mol) and Diabetes Control and Complications Trial (DCCT; %).
3
Blood Glucose Monitoring in ICU
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Blood glucose levels were read hourly for the first 48 hours. If patients were considered stable after 48 hours, readings were reduced to four times a day continuing until discharge from hospital. Blood samples were taken at the recipients' bedside using a capillary blood sample with a calibrated finger prick device (Abbot FreeStyle Precision Pro®, Witney, Oxon, UK). If the recipient was receiving a high dose of vasopressors, which affect peripheral perfusion, a blood sample from an arterial line was used instead. In the ICU, nurses adjusted the insulin doses, depending on the blood glucose level reading following the allocated protocol.
4
Continuous Glucose Monitoring in CAP Patients
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Continuous glucose monitoring was performed using iPro2® as the recorder and an Enlite® glucose sensor (Medtronic, Northridge, CA). The CGM system was inserted in the abdominal area according to the manufacturer’s guidelines. Recordings by CGM were fully blinded during hospitalization and therefore not used for in-hospital diabetes management. Standard POC capillary blood glucose measurements were performed by ward glucometers FreeStyle Precision Pro® (Abbot, Berkshire, UK) three times daily before main meals (7:00 AM, 12:00 AM, and 5:00 PM) to calibrate the CGMs. CGM data were collected from study enrollment until discharge. At least 24 h of CGM data were required to be included in the analyses.
At baseline, we collected clinical data (age, gender, comorbidities, CURB-65 score as a measurement of severity of CAP, early warning score, Charlson comorbidity index, and medications before admission) and standard blood work (hemoglobin A1c (HbA1c)). For the two groups receiving GCs, CGM data were analyzed only during GC exposure which was defined as the period between the first GC dose and 24 h (six half-lives for prednisolone) after GCs were stopped. Meal registration (timing and percentage of the amount of meal consumed at breakfast, lunch, and dinner) made it possible to calculate PPGE.
At baseline, we collected clinical data (age, gender, comorbidities, CURB-65 score as a measurement of severity of CAP, early warning score, Charlson comorbidity index, and medications before admission) and standard blood work (hemoglobin A1c (HbA1c)). For the two groups receiving GCs, CGM data were analyzed only during GC exposure which was defined as the period between the first GC dose and 24 h (six half-lives for prednisolone) after GCs were stopped. Meal registration (timing and percentage of the amount of meal consumed at breakfast, lunch, and dinner) made it possible to calculate PPGE.
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