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Laminb2 mab3536

Manufactured by Merck Group
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LaminB2 (MAB3536) is a mouse monoclonal antibody that recognizes human Lamin B2, a structural protein found in the cell nucleus. This product is intended for use in research applications.

Automatically generated - may contain errors

Lab products found in correlation

Irf1 sc 497, by Santa Cruz Biotechnology (2 mentions) Irf7 sc 13041, by Santa Cruz Biotechnology (2 mentions) α tubulin a01622, by GenScript (2 mentions) Zeb1 sc 25388, by Santa Cruz Biotechnology (2 mentions) Zeb2 sc 271984, by Santa Cruz Biotechnology (2 mentions) Odyssey blocking buffer, by LI COR (2 mentions) Pvdf membrane, by Merck Group (2 mentions) Odyssey infrared imaging system, by LI COR (2 mentions) Sds page, by GenScript (2 mentions)

2 protocols using laminb2 mab3536

1

Western Blot Analysis of Nuclear Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sucrose cushion-purified Nuclear proteins were used 49 (link). For Western blots, equal amounts of protein were resolved by 4–20% SDS-PAGE (Genscript) and transferred to PVDF membranes (Millipore). The membranes were blocked for 1 h at room temperature with Odyssey blocking buffer (LI-COR) and incubated overnight at 4 °C with primary antibodies. After incubation with IRDye 680/800-conjugated secondary antibodies, the membranes were scanned using the Odyssey Infrared Imaging system (LI-COR). The following antibodies were used: IRF1 (sc-497, Santa Cruz), IRF7 (sc-13041, Santa Cruz), ZEB1 (sc-25388, Santa Cruz), ZEB2 (sc-271984, Santa Cruz), α-Tubulin (A01622, Genscript), and LaminB2 (MAB3536, Chemicon).
Yang J., Tian B., Sun H., Garofalo R.P, & Brasier A.R. (2017). Epigenetic silencing of IRF1 dysregulates type III interferon responses to respiratory virus infection in epithelial to mesenchymal transition. Nature microbiology, 2, 17086.
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2

Western Blot Analysis of Nuclear Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sucrose cushion-purified Nuclear proteins were used 49 (link). For Western blots, equal amounts of protein were resolved by 4–20% SDS-PAGE (Genscript) and transferred to PVDF membranes (Millipore). The membranes were blocked for 1 h at room temperature with Odyssey blocking buffer (LI-COR) and incubated overnight at 4 °C with primary antibodies. After incubation with IRDye 680/800-conjugated secondary antibodies, the membranes were scanned using the Odyssey Infrared Imaging system (LI-COR). The following antibodies were used: IRF1 (sc-497, Santa Cruz), IRF7 (sc-13041, Santa Cruz), ZEB1 (sc-25388, Santa Cruz), ZEB2 (sc-271984, Santa Cruz), α-Tubulin (A01622, Genscript), and LaminB2 (MAB3536, Chemicon).
Yang J., Tian B., Sun H., Garofalo R.P, & Brasier A.R. (2017). Epigenetic silencing of IRF1 dysregulates type III interferon responses to respiratory virus infection in epithelial to mesenchymal transition. Nature microbiology, 2, 17086.
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