HEK293T and HEK293T-i-3CS-GLuc2 cells were seeded in confocal dishes (NEST). At 24 hours post seeding, cells were washed, fixed with 4% formaldehyde, permeabilized with 2% Triton-X100, blocked with PBS buffer containing 2% BSA and 5% normal goat serum (Beyotime), and immunostained with anti-Flag monoclonal antibodies and FITC-labeled secondary antibodies. After washing, cell nuclei were counterstained with Hoechst 33258 (Beyotime) and visualized on a PerkinElmer UltraView VOX system using a Nikon Ti microscope.
Ultraview vox system
Manufactured by Nikon
Sourced in Japan
The UltraView VOX system is a high-performance imaging solution designed for advanced life science research. It provides users with the ability to visualize and analyze cellular and subcellular structures and dynamics in living samples with high speed and resolution.
Automatically generated - may contain errors
2 protocols using ultraview vox system
1
Immunostaining Protocol for HEK293T Cells
2
Photomicrographic Imaging of DAB and Fluorescent-Stained Tissues
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Photomicrographs of DAB-stained tissue were taken using the ZEISS Axio Lab.A1 with an attached 6-megapixel ZEISS Axiocam 506 color camera using the 20x/0.4 objectives (ZEISS, Germany). For fluorescent-stained tissue, z-stack images (1 image taken per 1 μm of optical thickness) were captured via the Volocity 6.3 software using the Perkin-Elmer UltraVIEW VoX system, involving an inverted Ti Eclipse microscope (Nikon, Japan) with a CSU-X1 spinning disk confocal scanner (Yokogawa Electric Corporation, Japan), plan apochromatic 20x/0.75 objective (Nikon, Japan), and excitation lasers/emission filters of wavelength 488/525 and 561/615.
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