The employed strains, A. Baumannii strains A1 (minocycline-sensitive strain), A163 (minocycline-mediated strain), and A156 (minocycline-resistant strain), were clinically isolated from Zhangjiagang people’s hospital, Xuyi hospital, and Affiliated Hospital of Yangzhou University, respectively and preserved in the pathogenic microbiology laboratory of Yangzhou University while the strains were grown in Mueller–Hinton (MH) broth or on MH agar. Metformin were obtained from Macklin (Shanghai,China), minocycline were obtained form Solarbio (Beijing, China), other antibiotics were obtained from Sangon Biotech (Shanghai, China).
Metformin
Manufactured by Maclin Biochemical Technology
Sourced in China
Metformin is a pharmaceutical compound used in the treatment of type 2 diabetes. It is an oral antidiabetic drug that works by reducing the amount of glucose produced by the liver and increasing the body's sensitivity to insulin.
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Lab products found in correlation
2 protocols using metformin
1
Antibiotic Susceptibility of A. baumannii Strains
2
Multifunctional Nanoparticle Tumor Therapy
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NIR dye CyI (MW 776.5) was prepared in our laboratory. Egg yolk lecithin (PC-98T) was acquired from AVT Pharmaceutical Tech Co. (Shanghai, China). 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG2000) was purchased from Ruixi Bio-tech Co. (Xi’an, China). Metformin was purchased from Macklin. Singlet Oxygen Sensor Green (SOSG) was purchased from Meilunbio. DAPI solution, Methyl thiazolyl-tetrazolium (MTT), Annexin V-FITC/PI apoptosis staining kit were purchased from Solarbio (Beijing, China).
Mouse colon cancer cells (CT26) were purchased from Wuhan Procell Life Science & Technology. Co. Ltd. (Wuhan, China). Cells were cultured in Roswell Park Memorial Institute-1640 (RPMI-1640, Procell, USA) with 10% fetal bovine serum (Procell, USA) and 1% penicillin-streptomycin (Hyclone, USA). BALB/c mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China).
Mouse colon cancer cells (CT26) were purchased from Wuhan Procell Life Science & Technology. Co. Ltd. (Wuhan, China). Cells were cultured in Roswell Park Memorial Institute-1640 (RPMI-1640, Procell, USA) with 10% fetal bovine serum (Procell, USA) and 1% penicillin-streptomycin (Hyclone, USA). BALB/c mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China).
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