The animals were euthanized by using an overdose of sodium pentobarbital (0.5 mL/kg, Nembutal injection, Abbott Laboratories, USA) following general anesthesia, 12 weeks after implantation. The fixed specimens were examined by using a microcomputed tomography (micro-CT) system (µCT-80, Scanco Medical, Switzerland). Moreover, the trabecular number (Tb.N), bone mineral density (BMD), and bone volume fraction (BV/TV) in the bone defect were calculated by using the auxiliary histomorphometric software (Scanco Medical AG, Switzerland).
Auxiliary histomorphometric software
Manufactured by Scanco
Sourced in Switzerland
Auxiliary histomorphometric software is a tool that supports the analysis and quantification of microscopic tissue samples. It provides functionalities for image processing and measurement of various histological parameters, without interpretation or extrapolation of the intended use.
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Lab products found in correlation
2 protocols using auxiliary histomorphometric software
1
Micro-CT Analysis of Bone Healing
2
Micro-CT Analysis of Bone Regeneration
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At 8 weeks after the operation, the rats in each group were sacrificed using an overdose of pentobarbital. The calvarias were fixed in a 4% phosphate-buffered formalin solution and then detected by a microcomputed tomography (micro-CT) system (μCT-80, Scanco Medical AG, Switzerland) as described in the previous study (Zhou et al., 2017 (link)). The segmentation of bone tissue from the CS/SF and SrCS/SF was carried out by the threshold segmentation method, while the selected bone grey threshold range was 120–255. Moreover, the bone mineral density (BMD) and the trabecular thickness (Tb. Th) of the bone defects were calculated by auxiliary histomorphometric software (Scanco Medical AG, Switzerland). All experiments were performed in triplicate.
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