Fluka kieselgel 60 f254

Manufactured by Merck Group

Sourced in Germany

Fluka Kieselgel 60 F254 is a silica gel-based chromatographic material used for thin-layer chromatography (TLC) and column chromatography. It is characterized by a particle size of 63-200 μm and the presence of a fluorescent indicator (F254) that allows for easy visualization of separated compounds under UV light.

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Lab products found in correlation

Laborota 4000, by Heidolph (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Deuterated solvent, by Merck Group (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt dimethyl sulfoxide dmso, by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) Rpmi 1640 medium, by Merck Group (1 mentions) Marsxpress, by CEM Corporation (1 mentions)

Spelling variants of this product

Kieselgel 60 F254 (259 citations) Kieselgel 60 (220 citations)

3 protocols using fluka kieselgel 60 f254

Solvents and Chromatographic Techniques

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HPLC grade-solvents were supplied by Honeywell (Seelze, Germany), while analytical grade-solvents by PanReac (Darmstadt, Germany). The evaporation procedures were performed under reduced pressure using a Heidolph Laborota 4000 instrument (Heidolph Instruments GmbH & Co., Schwabach, Germany).
Analytical thin-layer chromatography (TLC) was carried out on silica gel precoated glass-backed plates (Fluka Kieselgel 60 F254, Merck, Darmstadt, Germany). The detection was conducted with UV light (λ = 254 nm).
Flash chromatography was performed with silica gel 60 (particle size 230–400 mesh) purchased from Nova Chimica (Cinisello Balsamo, Italy).
Deuterated solvent and all the reactants were purchased from Sigma Aldrich (Milan, Italy).

Cavalloro V., Russo K., Vasile F., Pignataro L., Torretta A., Donini S., Semrau M.S., Storici P., Rossi D., Rapetti F., Brullo C., Parisini E., Bruno O, & Collina S. (2020). Insight into GEBR-32a: Chiral Resolution, Absolute Configuration and Enantiopreference in PDE4D Inhibition. Molecules, 25(4), 935.

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Phytochemical Analysis of Chlorogenic Acid

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Solvents for both HPLC (HPLC grade) and extraction procedures (analytical grade) were supplied by Carlo Erba (Milan, Italy). Chlorogenic acid was purchased from Indena S.p.A. (Milan, Italy).
Analytical thin-layer chromatography (TLC) was carried out on silica gel precoated glass-backed plates (Fluka Kieselgel 60 F254, Merck, Darmstadt, Germany) and visualized by UV light or ceric ammonium molibdate (IV) stain (Hanessian’s Stain).
Flash chromatography was performed with silica gel 60 (particle size 230–400 mesh) purchased from Nova Chimica (Cinisello Balsamo, Italy).
Deuterated solvent for NMR spectroscopy, RPMI-1640 Medium, Phosphate Buffered Saline (PBS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethylsulfoxide (DMSO) were purchased from Sigma Aldrich (Milan, Italy).
Fetal bovine serum (FBS; GibcoR) was purchased from ThermoFisher Scientific (Lisbon, Portugal).

Malacrida A., Cavalloro V., Martino E., Cassetti A., Nicolini G., Rigolio R., Cavaletti G., Mannucci B., Vasile F., Giacomo M.D., Collina S, & Miloso M. (2019). Anti-Multiple Myeloma Potential of Secondary Metabolites from Hibiscus sabdariffa. Molecules, 24(13), 2500.

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Extraction of Phytochemicals from H. sabdariffa

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HPLC grade solvents were supplied by Honeywell (Germany), while analytical grade solvents were supplied by PanReac (Germany). The evaporation procedures were performed under reduced pressure using a Heidolph Laborota 4000 instrument (Heidolph Instruments GmbH & Co., Schwabach, Germany).
Analytical thin-layer chromatography (TLC) was carried out on silica gel pre-coated glass-backed plates (Fluka Kieselgel 60 F254, Merck, Darmstadt, Germany). The detection was conducted with UV light or ceric ammonium molybdate (IV) stain (Hanessian’s Stain).
Flash chromatography was performed with silica gel 60 (particle size 230–400 mesh) purchased from Nova Chimica (Cinisello Balsamo, Italy).
Fetal bovine serum (FBS; Gibco) was purchased from ThermoFisher Scientific (Lisbon, Portugal).
H. sabdariffa calyces were powdered using a blade mill (A10 IKA-Werke GmbH & Co., Staufen, Germany) and then extracted exploiting a multimode microwave apparatus (MARSX press, CEM Corporation, Matthews, NC, USA).

Malacrida A., Cavalloro V., Martino E., Costa G., Ambrosio F.A., Alcaro S., Rigolio R., Cassetti A., Miloso M, & Collina S. (2021). Anti-Multiple Myeloma Potential of Secondary Metabolites from Hibiscus sabdariffa—Part 2. Molecules, 26(21), 6596.

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