Control igg

Manufactured by Diagenode

Control IgG is a laboratory reagent used as a control in various immunological assays. It is a purified immunoglobulin G (IgG) from a non-immune source, typically a non-human species, that serves as a negative control to help establish a baseline or reference point for experimental measurements.

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Lab products found in correlation

Ab4729, by Abcam (1 mentions) Anti h3k4me3, by Diagenode (1 mentions) Anti brd4, by Fortis Life Sciences (1 mentions) Anti pol 2, by Cell Signaling Technology (1 mentions) Anti h3k27ac, by Abcam (1 mentions) Ideal chip seq kit for transcription factors, by Diagenode (1 mentions) Platinum sybr green qpcr supermix udg, by Thermo Fisher Scientific (1 mentions) Lowcell chip kit, by Diagenode (1 mentions)

Spelling variants of this product

Rabbit IgG control antibody (2 citations)

2 protocols using control igg

Chromatin Profiling of JQ1 Responses

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LN-2683GS cells were treated with 1 µM JQ1, DMSO, and 1000 units/mL IFN-α for 2 hours. U87MG were treated with 1 µM JQ1 or DMSO for 24 hours. Cells were cross-linked and ChIP was performed using iDeal ChIP-seq kit for Transcription Factors (Diagenode) as detailed in the Supplementary Methods, using the following antibodies: anti-Pol II (Cell Signaling, D8L4Y, 1:50), anti-BRD4 (Bethyl Laboratories, A301, 1:60), anti-H3K4me3 (Diagenode, C15410003, 1:300), anti-H3K27ac (Abcam, ab4729, 1:300 or 1:600), control IgG (1:600, Diagenode).

Gusyatiner O., Bady P., Pham M.D., Lei Y., Park J., Daniel R.T., Delorenzi M, & Hegi M.E. (2021). BET inhibitors repress expression of interferon-stimulated genes and synergize with HDAC inhibitors in glioblastoma. Neuro-Oncology, 23(10), 1680-1692.

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Assessing H3K27me3 Chromatin Modifications

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We fixed 5 × 10 5 PBMCs in 1% formaldehyde for 10 min. Chromatin immunoprecipitation (ChIP) was performed using the LowCell# ChIP kit (Diagenode, Liège, Belgium) using chromatin from 3,000 to 8,000 cells per reaction. For the immunoprecipitation, an antibody against H3K27me3 (9756; Cell Signaling Technology, Danvers, Mass., USA) was used. Control IgG was supplied by Diagenode. The ChIP was evaluated by qPCR using 'platinum SYBR Green qPCRSuperMix-UDG' (Invitrogen, Carlsbad, Calif., USA). Results were normalized to 1%.

Jungebluth P., Holzgraefe B., Lim M.L., Duru A.D., Lundin V., Heldring N., Wiklander O.P.B., Nordin J.Z., Chrobok M., Roderburg C., Sjöqvist S., Anderstam B., Beltrán Rodríguez A., Haag J.C., Gustafsson Y., Roddewig K.G., Jones P., Wood M.J.A., Luedde T., Teixeira A.I., Hermanson O., Winqvist O., Kalzén H., El Andaloussi S., Alici E, & Macchiarini P. (2015). Autologous Peripheral Blood Mononuclear Cells as Treatment in Refractory Acute Respiratory Distress Syndrome. Respiration; international review of thoracic diseases, 90(6).

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