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Discovery ultra automated processor

Manufactured by Roche
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The Discovery Ultra is an automated processor designed for high-throughput sample preparation and handling. It is capable of performing various laboratory operations such as pipetting, mixing, and incubation to facilitate efficient sample processing. The core function of the Discovery Ultra is to streamline and automate routine tasks, enabling researchers to increase productivity and efficiency in their laboratory workflows.

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Lab products found in correlation

Chromomap dab kit, by Roche (5 mentions) Impress vr anti rabbit igg polymer, by Vector Laboratories (4 mentions) U864yfa140 4 cb2093 np 1, by GenScript (2 mentions) U864yfa140 4 cb2093 np 1, by Vector Laboratories (2 mentions) Impress vr anti rabbit igg polymer, by Roche (1 mentions) Impress vr, by Vector Laboratories (1 mentions)

Spelling variants of this product

Discovery Ultra (119 citations) Discovery ULTRA automated (4 citations) Discovery Ultra processor (3 citations)

5 protocols using discovery ultra automated processor

1

SARS-CoV-2 Immunohistochemistry Staining

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Tissues were fixed in 10% neutral buffered formalin for a minimum of 7 days with two changes of formalin. Tissues were placed in cassettes and processed with a Sakura VIP-6 Tissue Tek, on a 12-h automated schedule, using a graded series of ethanol, xylene, and ParaPlast Extra. Embedded tissues are sectioned at 5 μm and dried overnight at 42°C before staining.
Specific anti-CoV immunoreactivity was detected using GenScript U864YFA140-4/CB2093 NP-1 at a 1:1,000 dilution. The secondary antibody is the Vector Laboratories ImPress VR anti-rabbit IgG polymer (cat# MP-6401). The tissues were then processed for immunohistochemistry using the Discovery Ultra automated processor (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat#760-159).
Hansen F., Meade-White K., Clancy C., Rosenke R., Okumura A., Hawman D.W., Feldmann F., Kaza B., Jarvis M.A., Rosenke K, & Feldmann H. (2022). SARS-CoV-2 reinfection prevents acute respiratory disease in Syrian hamsters but not replication in the upper respiratory tract. Cell Reports, 38(11), 110515.
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2

Immunohistochemical Detection of SARS-CoV-2 and Immune Markers

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Necropsies and tissue sampling were performed according to IBC-approved protocols. Tissues were fixed for a minimum of 7 days in 10% neutral buffered formalin with 2 changes. Tissues were placed in cassettes and processed with a Sakura VIP-6 Tissue Tek, on a 12-hour automated schedule, using a graded series of ethanol, xylene, and PureAffin. Prior to staining, embedded tissues were sectioned at 5 μm and dried overnight at 42°C. Using GenScript U864YFA140–4/CB2093 NP-1 (1:1000) specific anti-CoV immunoreactivity was detected using the Vector Laboratories ImPress VR anti-rabbit IgG polymer (# MP-6401) as secondary antibody. The tissues were then processed using the Discovery Ultra automated processor (Ventana Medical Systems) with a ChromoMap DAB kit Roche Tissue Diagnostics (#760–159). Anti-CD3 immunoreactivity was detected utilizing a primary antibody from Roche Tissue Diagnostics predilute (#790–4341), secondary antibody from Vector Laboratories ImPress VR anti-rabbit IgG polymer (# MP-6401) and visualized using the ChromoMap DAB kit from Roche Tissue Diagnostics (#760–159). Anti-PAX5 immunoreactivity was detected utilizing a primary antibody from Novus Biologicals at 1:500 (#NBP2–38790), secondary antibody from Vector Laboratories ImPress VR anti-rabbit IgG polymer (# MP-6401) and visualized using the ChromoMap DAB kit from Roche Tissue Diagnostics (#760–159).
Port J.R., Yinda C.K., Riopelle J.C., Weishampel Z.A., Saturday T.A., Avanzato V.A., Schulz J.E., Holbrook M.G., Barbian K., Perry-Gottschalk R., Haddock E., Martens C., Shaia C.I., Lambe T., Gilbert S.C., van Doremalen N, & Munster V.J. (2022). Infection- or vaccine mediated immunity reduces SARS-CoV-2 transmission, but increases competitiveness of Omicron in hamsters. bioRxiv.
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3

SARS-CoV-2 Immunohistochemical Tissue Analysis

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Necropsies and tissue sampling were performed according to IBC-approved protocols. Tissues were fixed for a minimum of 7 days in 10% neutral buffered formalin with 2 changes. Tissues were placed in cassettes and processed with a Sakura VIP-6 Tissue Tek on a 12 hr automated schedule using a graded series of ethanol, xylene, and ParaPlast Extra. Prior to staining, embedded tissues were sectioned at 5 µm and dried overnight at 42 °C. Using GenScript U864YFA140-4/CB2093 NP-1 (1:1000), specific anti-CoV immunoreactivity was detected using the Vector Laboratories ImPress VR anti-rabbit IgG polymer (# MP-6401) as secondary antibody. The tissues were then processed using the Discovery Ultra automated processor (Ventana Medical Systems) with a ChromoMap DAB kit Roche Tissue Diagnostics (#760–159).
Port J.R., Morris D.H., Riopelle J.C., Yinda C.K., Avanzato V.A., Holbrook M.G., Bushmaker T., Schulz J.E., Saturday T.A., Barbian K., Russell C.A., Perry-Gottschalk R., Shaia C., Martens C., Lloyd-Smith J.O., Fischer R.J, & Munster V.J. (2024). Host and viral determinants of airborne transmission of SARS-CoV-2 in the Syrian hamster. eLife, 12, RP87094.
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4

Histopathological and Immunohistochemical Analysis of SARS-CoV-2 in Rhesus Macaques

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Histopathology and IHC were performed on rhesus macaque tissues. After fixation for a minimum of 7 days in 10% neutral-buffered formalin and embedding in paraffin, tissue sections were stained with H&E. Tissues were placed in cassettes and processed with a Sakura VIP-6 Tissue Tek on a 12-hour automated schedule, using a graded series of ethanol, xylene, and ParaPlast Extra. Embedded tissues were sectioned at 5 μm and dried overnight at 42°C before staining. Specific anti-CoV immunoreactivity was detected using GenScript U864YFA140-4/CB2093 NP-1 at a 1:1000 dilution. The secondary antibody was an anti–rabbit IgG polymer from Vector Laboratories ImPress VR. Tissues were then processed for IHC using the Discovery Ultra automated processor (Ventana Medical Systems) with a ChromoMap DAB Kit (Roche Tissue Diagnostics). Stained slides were analyzed by a board-certified veterinary pathologist.
Rosenke K., Jarvis M.A., Feldmann F., Schwarz B., Okumura A., Lovaglio J., Saturday G., Hanley P.W., Meade-White K., Williamson B.N., Hansen F., Perez-Perez L., Leventhal S., Tang-Huau T.L., Callison J., Haddock E., Stromberg K.A., Scott D., Sewell G., Bosio C.M., Hawman D., de Wit E, & Feldmann H. (2020). Hydroxychloroquine prophylaxis and treatment is ineffective in macaque and hamster SARS-CoV-2 disease models. JCI Insight, 5(23), e143174.
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5

Immunohistochemical Detection of SARS-CoV-2 Nucleoprotein

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Necropsies and tissue sampling were performed according to IBC-approved protocols. Tissues were fixed for a minimum of 7 days in 10% neutral buffered formalin with 2 changes. Tissues were placed in cassettes and processed with a Sakura VIP-6 Tissue Tek on a 12-h automated schedule using a graded series of ethanol, xylene, and ParaPlast Extra. Prior to staining, embedded tissues were sectioned at 5 μm and dried overnight at 42°C. Using GenScript U864YFA140-4/CB2093 NP-1 (1:1000), specific anti-CoV immunoreactivity was detected using the Vector Laboratories ImPress VR anti-rabbit IgG polymer (# MP-6401) as secondary antibody. The tissues were then processed using the Discovery Ultra automated processor (Ventana Medical Systems) with a ChromoMap DAB kit Roche Tissue Diagnostics (#760-159).
Port J.R., Morris D.H., Riopelle J.C., Yinda C.K., Avanzato V.A., Holbrook M.G., Bushmaker T., Schulz J.E., Saturday T.A., Barbian K., Russell C.A., Perry-Gottschalk R., Shaia C.I., Martens C., Lloyd-Smith J.O., Fischer R.J, & Munster V.J. (2023). Host and viral determinants of airborne transmission of SARS-CoV-2 in the Syrian hamster. bioRxiv.
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