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2 protocols using l 3 4 dihydroxyphenylalanine l dopa

1

Tyrosinase Inhibition Assay Protocol

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Mushroom tyrosinase, chlorogenic acid, epicatechin, schisandrin, and sodium phosphate monobasic were purchased from Sigma-Aldrich (St. Louis, MO, USA). L-3,4-Dihydroxyphenylalanine (L-dopa) and kojic acid were purchased from Acros (New Jersey, USA). Acetonitrile was purchased from Aencore (Surrey Hills, Australia). Sodium phosphate dibasic anhydrous was purchased from J. T. Baker (Petaling Jaya, Selangor, Malaysia). Methanol was purchased from Merck (Darmstadt, Germany). RVLE (Elastvein®) was purchased from Healthmate (Changhua, Taiwan). Acetic acid was purchased from Panreac (Barcelona, Spain), and gallic acid was purchased from Alfa Aesar (Ward Hill, MA, USA). Rutin was purchased from Extrasynthese (Genay, France). Resveratrol was purchased from TCI (Tokyo, Japan).
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2

Biochemical Reagent Sourcing Protocol

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Anthrone, L-3,4-dihydroxyphenylalanine (L-DOPA), and vanillin reagents were purchased from Acros Organics (Fair Lawn, NJ). Bicinchoninic acid, chloroform, chlorothalonil, copper sulfate, sulfuric acid, Triton X-100, and glucose were purchased from Sigma Aldrich (St. Louis, MO). Chymotrypsin and o-dianisidine were purchased from MP Biomedicals (Solon, OH). Horseradish peroxidase was purchased from Novex Life Technologies (Grand Island, NY).
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